7 Questions 11 Answers 0 Followers
Questions related from Chenglong Yu
Dear all, I am working on real time imaging of cultured neurons. Currently, I want to distinguish presynaptic boutons from extra-synaptic areas in live-cell imaging. My advisor suggest to incubate...
04 April 2017 1,547 4 View
Hi guys, I am working on live-cell imaging of primary cultured neurons. I want to identify the formation of synapses in my live-imaging. I doubt the transfection of synaptic markers as they may go...
07 July 2016 8,525 7 View
Hi, I am looking for good fluorescent markers to identify pre-/postsynaptic sites. Something like PSD95-GFP and synaptophysin-mCherry (Syp–Cherry). Any suggestions will be appreciated. Besides,...
02 February 2016 1,043 0 View
Hi guys, I am looking for a binding partner for my molecule. It should be a new one as no one knows what it is. I feel that there should have a binding partner. But it is just a kind of...
12 December 2015 4,725 7 View
Dear all, I am straggling with Ca transfection on neurons. I heared that antibiotics like Penicillin will reduce the transfection efficiency. My transfection medium do not contain antibiotics, but...
04 April 2015 5,713 4 View
Dear all, I am working on a truncated TrkB receptor, TrkB.T1. Now, I need to increase or inhibit its activity in cultured neurons. Do you guys know any good agonist or activator for TrkB.T1? Or,...
03 March 2015 3,646 2 View
I would like to quantify the expression of a protein in neurons. However, it is also highly expressed in glia. How can I exclude the glia protein expression? I mean to test the protein levels...
03 March 2015 6,970 5 View
