Live-imaging for synaptogenesis?

Hi guys, I am working on live-cell imaging of primary cultured neurons. I want to identify the formation of synapses in my live-imaging. I doubt the transfection of synaptic markers as they may go...

06 July 2016 8,491 7 View

Fluorescent markers for pre-/postsynaptic site

01 February 2016 1,020 0 View

How can I screen an unknown partner of a known protein?

Hi guys, I am looking for a binding partner for my molecule. It should be a new one as no one knows what it is. I feel that there should have a binding partner. But it is just a kind of...

11 December 2015 4,705 7 View

Does antibiotics affect the efficiency for Ca transfection?

Dear all, I am straggling with Ca transfection on neurons. I heared that antibiotics like Penicillin will reduce the transfection efficiency. My transfection medium do not contain antibiotics, but...

03 April 2015 5,690 4 View

Is there any antagonist or agonist for TrkB.T1?

Dear all, I am working on a truncated TrkB receptor, TrkB.T1. Now, I need to increase or inhibit its activity in cultured neurons. Do you guys know any good agonist or activator for TrkB.T1? Or,...

02 March 2015 3,612 2 View

How can I test for protein expression only in neurons?

I would like to quantify the expression of a protein in neurons. However, it is also highly expressed in glia. How can I exclude the glia protein expression? I mean to test the protein levels...

02 March 2015 6,950 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View