hi all , we have Naviuos FC from Beckan Coulter.
I had got alarm saying ( sample pressure error)
but there was normal aquisition , have any one expirence the same error ?
How long do they take before initial disicion ?
04 May 2019 5,762 2 View
what is the minmum count to perform paired sample gene expression assay if my cases have pre and post reading eg I have 4 cases with low expression and 6 cases of high expression baseline and...
11 December 2018 5,711 1 View
I use free software from image j to analyse microscopic pictures , but , I cant use the IHC tool properly as well as I cant undestand some features. do any one have tutorial videos to use the program?
03 April 2018 3,393 4 View
I wanr to compare differnt categerical varibles on gene expression ( hig , low) can I use linear rigression ?
02 March 2018 6,597 3 View
How to compare un normalized RT PCR DATA Between cases and control (relative quatitation)
01 February 2018 8,213 3 View
hellow all When to use Delta CT vs DD CT in gene expression assay
31 December 2017 9,412 3 View
Hi all I use total RNA EXTRACTION KIT ( QIAGEN). THEN I USE SYBR GREEN BASE RT PCR to antiquate HOTAIR LncRNA What is the accepted change in Tm between run of my product
11 December 2017 2,746 1 View
I use 1ug of total RNA in cDNA reaction using revert aid first starnd cDNA synthesis then I use 1ul in qpcr reaction (10 ul volume) My HK gene usually less than 10 I measured thre cDNA using...
10 November 2017 7,558 4 View
Hi all I use a sybr green based assay . i sawproduct at ct 34 in NTC any recommendation ??
10 November 2017 1,919 8 View
Hi all Any explainaition of why i got very ealy CT in b actin HK gene I use sybr green based assay with ROX passive refrence dye . I got early ct of HK gene ( 11-14). is that accepted ?
08 September 2017 9,341 0 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...
25 July 2024 2,309 5 View
Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...
25 July 2024 9,058 4 View
Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...
22 July 2024 2,257 0 View