hi all , we have Naviuos FC from Beckan Coulter.
I had got alarm saying ( sample pressure error)
but there was normal aquisition , have any one expirence the same error ?
How long do they take before initial disicion ?
04 May 2019 5,635 2 View
what is the minmum count to perform paired sample gene expression assay if my cases have pre and post reading eg I have 4 cases with low expression and 6 cases of high expression baseline and...
11 December 2018 5,594 1 View
I use free software from image j to analyse microscopic pictures , but , I cant use the IHC tool properly as well as I cant undestand some features. do any one have tutorial videos to use the program?
03 April 2018 3,275 4 View
I wanr to compare differnt categerical varibles on gene expression ( hig , low) can I use linear rigression ?
02 March 2018 6,478 3 View
How to compare un normalized RT PCR DATA Between cases and control (relative quatitation)
01 February 2018 8,016 3 View
hellow all When to use Delta CT vs DD CT in gene expression assay
31 December 2017 9,033 3 View
Hi all I use total RNA EXTRACTION KIT ( QIAGEN). THEN I USE SYBR GREEN BASE RT PCR to antiquate HOTAIR LncRNA What is the accepted change in Tm between run of my product
11 December 2017 2,644 1 View
I use 1ug of total RNA in cDNA reaction using revert aid first starnd cDNA synthesis then I use 1ul in qpcr reaction (10 ul volume) My HK gene usually less than 10 I measured thre cDNA using...
10 November 2017 7,413 4 View
Hi all I use a sybr green based assay . i sawproduct at ct 34 in NTC any recommendation ??
10 November 2017 1,768 8 View
Hi all Any explainaition of why i got very ealy CT in b actin HK gene I use sybr green based assay with ROX passive refrence dye . I got early ct of HK gene ( 11-14). is that accepted ?
08 September 2017 9,244 0 View
When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...
03 March 2021 4,892 5 View
We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...
03 March 2021 7,975 3 View
We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...
02 March 2021 6,036 1 View
Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...
02 March 2021 4,910 3 View
Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...
02 March 2021 1,999 5 View
(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...
02 March 2021 3,309 2 View
I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...
02 March 2021 5,829 1 View
Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...
01 March 2021 8,235 2 View
I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...
01 March 2021 6,985 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View