Anyone had experience in publication in Journal of applied genetics?

How long do they take before initial disicion ?

04 May 2019 5,635 2 View

Paired samples statistics?

what is the minmum count to perform paired sample gene expression assay if my cases have pre and post reading eg I have 4 cases with low expression and 6 cases of high expression baseline and...

11 December 2018 5,594 1 View

Flow cytometry alarming messages?

hi all , we have Naviuos FC from Beckan Coulter. I had got alarm saying ( sample pressure error) but there was normal aquisition , have any one expirence the same error ?

03 April 2018 8,621 0 View

Statistical method of data represntaton?

I wanr to compare differnt categerical varibles on gene expression ( hig , low) can I use linear rigression ?

02 March 2018 6,478 3 View

Un normalised q pcr ?

How to compare un normalized RT PCR DATA Between cases and control (relative quatitation)

01 February 2018 8,016 3 View

Delta CT in qPCR Reactions?

hellow all When to use Delta CT vs DD CT in gene expression assay

31 December 2017 9,033 3 View

LncRNA METING TEMPRATURE?

Hi all I use total RNA EXTRACTION KIT ( QIAGEN). THEN I USE SYBR GREEN BASE RT PCR to antiquate HOTAIR LncRNA What is the accepted change in Tm between run of my product

11 December 2017 2,644 1 View

What the optimum concentration of cDNA in rt pcr?

I use 1ug of total RNA in cDNA reaction using revert aid first starnd cDNA synthesis then I use 1ul in qpcr reaction (10 ul volume) My HK gene usually less than 10 I measured thre cDNA using...

10 November 2017 7,413 4 View

PCR amplified product?

Hi all I use a sybr green based assay . i sawproduct at ct 34 in NTC any recommendation ??

10 November 2017 1,768 8 View

Early ct in house keeping gene?

Hi all Any explainaition of why i got very ealy CT in b actin HK gene I use sybr green based assay with ROX passive refrence dye . I got early ct of HK gene ( 11-14). is that accepted ?

08 September 2017 9,244 0 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

How to threshold an image despite bright lighting, using ImageJ/Fiji?

Hi everyone, I am using Fiji to determine the percentage of the image covered by leaves, branches, or trunk, but i've encountered some difficulty when the sun hits parts of my image like the one...

27 February 2021 1,194 3 View

Trouble with Charting with Google Earth Engine (Technical)?

Hello. I have a problem with the charting in google earth engine. I created an image collection by combining 5 image collections of five parameters and using ee.Join.inner ( ) and .apply ( )...

26 February 2021 7,084 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View