23 Questions 53 Answers 0 Followers
Questions related from Fatma Abdel Wahab
How long do they take before initial disicion ?
05 May 2019 5,723 2 View
what is the minmum count to perform paired sample gene expression assay if my cases have pre and post reading eg I have 4 cases with low expression and 6 cases of high expression baseline and...
12 December 2018 5,669 1 View
I use free software from image j to analyse microscopic pictures , but , I cant use the IHC tool properly as well as I cant undestand some features. do any one have tutorial videos to use the program?
04 April 2018 3,365 4 View
hi all , we have Naviuos FC from Beckan Coulter. I had got alarm saying ( sample pressure error) but there was normal aquisition , have any one expirence the same error ?
04 April 2018 8,701 0 View
I wanr to compare differnt categerical varibles on gene expression ( hig , low) can I use linear rigression ?
03 March 2018 6,572 3 View
How to compare un normalized RT PCR DATA Between cases and control (relative quatitation)
02 February 2018 8,139 3 View
hellow all When to use Delta CT vs DD CT in gene expression assay
01 January 2018 9,272 3 View
Hi all I use total RNA EXTRACTION KIT ( QIAGEN). THEN I USE SYBR GREEN BASE RT PCR to antiquate HOTAIR LncRNA What is the accepted change in Tm between run of my product
12 December 2017 2,714 1 View
I use 1ug of total RNA in cDNA reaction using revert aid first starnd cDNA synthesis then I use 1ul in qpcr reaction (10 ul volume) My HK gene usually less than 10 I measured thre cDNA using...
11 November 2017 7,497 4 View
Hi all I use a sybr green based assay . i sawproduct at ct 34 in NTC any recommendation ??
11 November 2017 1,882 8 View
Hi all Any explainaition of why i got very ealy CT in b actin HK gene I use sybr green based assay with ROX passive refrence dye . I got early ct of HK gene ( 11-14). is that accepted ?
09 September 2017 9,314 0 View
Hi all any one knowa how to export FCS files. from Beckman Coulter Navious ?
09 September 2017 8,288 4 View
Hi all I Had an experiment on ABL 7500 fast , Using Meltdoctor Master mix But the assistant had choosed to Run fast experment Now , Can I reentre the plate , Or better to discard ?
08 August 2017 8,358 0 View
Hi all has any one tried PCR data analysis through free online thermofisher cloud Regarding data privacy
08 August 2017 4,014 0 View
I was doing a mutation screening experiment using meltdoctor master mix. 18 cases. In two cases no flurcence was observed any explaination? NB these cases was used in another expriment and were...
08 August 2017 5,147 5 View
Hi all I amworking on LncRNA gene expression after amplification the house keeping gene give CT about 19- 22 But my gene of interest give very high CT above 40 What? should I do
08 August 2017 298 6 View
plz would you recmmend a method for Monoclonal antibody titation for flow cytometry?
08 August 2017 4,414 4 View
Have any one tried to extract RNA from 72 hour old sample
04 April 2017 7,617 3 View
training on flow cytometry
02 February 2017 748 4 View
PleaseI need to stablish atouch down technique in PCR experamnet , any one can help
01 January 1970 5,894 0 View
hello Iam doing an Mutation screening experiments byHRM . the mutant variants should be then sequenced Should I preserve the amplicon at -20 or I should run another reaction immediately before...
01 January 1970 6,156 3 View
Have anyone experienced the spinger transfer desk
01 January 1970 1,658 1 View
How long I could preserve RNA afyer extraction in -20?
01 January 1970 4,782 1 View
