Hi all Any explainaition of why i got very ealy CT in b actin HK gene
I use sybr green based assay with ROX passive refrence dye .
I got early ct of HK gene ( 11-14).
is that accepted ?
How long do they take before initial disicion ?
04 May 2019 5,762 2 View
what is the minmum count to perform paired sample gene expression assay if my cases have pre and post reading eg I have 4 cases with low expression and 6 cases of high expression baseline and...
11 December 2018 5,711 1 View
hi all , we have Naviuos FC from Beckan Coulter. I had got alarm saying ( sample pressure error) but there was normal aquisition , have any one expirence the same error ?
03 April 2018 8,735 0 View
I use free software from image j to analyse microscopic pictures , but , I cant use the IHC tool properly as well as I cant undestand some features. do any one have tutorial videos to use the program?
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I wanr to compare differnt categerical varibles on gene expression ( hig , low) can I use linear rigression ?
02 March 2018 6,597 3 View
How to compare un normalized RT PCR DATA Between cases and control (relative quatitation)
01 February 2018 8,213 3 View
hellow all When to use Delta CT vs DD CT in gene expression assay
31 December 2017 9,412 3 View
Hi all I use total RNA EXTRACTION KIT ( QIAGEN). THEN I USE SYBR GREEN BASE RT PCR to antiquate HOTAIR LncRNA What is the accepted change in Tm between run of my product
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I use 1ug of total RNA in cDNA reaction using revert aid first starnd cDNA synthesis then I use 1ul in qpcr reaction (10 ul volume) My HK gene usually less than 10 I measured thre cDNA using...
10 November 2017 7,558 4 View
Hi all I use a sybr green based assay . i sawproduct at ct 34 in NTC any recommendation ??
10 November 2017 1,919 8 View
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