Early ct in house keeping gene?

Fatma Abdel Wahab @Fatma_Abdel_Wahab

09 September 2017 0 9K Report

Hi all Any explainaition of why i got very ealy CT in b actin HK gene

I use sybr green based assay with ROX passive refrence dye .

I got early ct of HK gene ( 11-14).

is that accepted ?

Badges
Science method

More Fatma Abdel Wahab's questions See All

Anyone had experience in publication in Journal of applied genetics?

How long do they take before initial disicion ?

04 May 2019 5,635 2 View

Paired samples statistics?

what is the minmum count to perform paired sample gene expression assay if my cases have pre and post reading eg I have 4 cases with low expression and 6 cases of high expression baseline and...

11 December 2018 5,594 1 View

Flow cytometry alarming messages?

hi all , we have Naviuos FC from Beckan Coulter. I had got alarm saying ( sample pressure error) but there was normal aquisition , have any one expirence the same error ?

03 April 2018 8,621 0 View

Image analysis by image J?

I use free software from image j to analyse microscopic pictures , but , I cant use the IHC tool properly as well as I cant undestand some features. do any one have tutorial videos to use the program?

03 April 2018 3,275 4 View

Statistical method of data represntaton?

I wanr to compare differnt categerical varibles on gene expression ( hig , low) can I use linear rigression ?

02 March 2018 6,478 3 View

Un normalised q pcr ?

How to compare un normalized RT PCR DATA Between cases and control (relative quatitation)

01 February 2018 8,016 3 View

Delta CT in qPCR Reactions?

hellow all When to use Delta CT vs DD CT in gene expression assay

31 December 2017 9,033 3 View

LncRNA METING TEMPRATURE?

Hi all I use total RNA EXTRACTION KIT ( QIAGEN). THEN I USE SYBR GREEN BASE RT PCR to antiquate HOTAIR LncRNA What is the accepted change in Tm between run of my product

11 December 2017 2,644 1 View

What the optimum concentration of cDNA in rt pcr?

I use 1ug of total RNA in cDNA reaction using revert aid first starnd cDNA synthesis then I use 1ul in qpcr reaction (10 ul volume) My HK gene usually less than 10 I measured thre cDNA using...

10 November 2017 7,413 4 View

PCR amplified product?

Hi all I use a sybr green based assay . i sawproduct at ct 34 in NTC any recommendation ??

10 November 2017 1,768 8 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View