Special issue on PLS-SEM in Mathematics. Are you interested?

21 February 2021 5,601 3 View

How to properly compare two simulation methods?

I have two methods for doing Monte Carlo simulations. With both of them I have run serveral simulations and got the mean and variance of their results. I would like to determine whether both...

20 November 2020 5,732 5 View

Can you investigate psychometric properties of a questionnaire when the factor analysis is not-converging?

Dear All, First of all I hope everybody is doing good in these weird times. Second, I also have a question regarding a non converging factormodel. I translated a questionnaire from English to...

18 November 2020 9,367 4 View

How can I calculate percent concordant in Stata 14?

I did a logistic regression and now I need to know how to compute concordant pairs of the predicted probability of my Y variable. I want to do this because I need to know model fit. I did the...

20 October 2020 7,819 1 View

How to simulate in EnergyPlus after modifying the source code?

I've changed some equations in one of the .cc files of the source code in EnergyPlus (downloaded from GitHub), but I do know know how to implement that file in the EnergyPlus installation folder...

13 September 2020 4,539 1 View

How to use Factor Scores to create an index?

Hello all, I have used Principal Component Analysis to create a new variable that is like an index of a personal characteristic. The factor loadings of the variables used to create this index...

11 August 2020 5,182 6 View

Should there be sex education in schools and institutes about female menstruation?

02 July 2020 5,724 13 View

What percentage would have decreased murders for gender violence in Spain in ten years?

Some of the factors for this to be possible would be: Complete secondary education for girls (and boys); delay the age of marriages to 18 years; women's economic autonomy and access to skills...

11 March 2020 3,837 3 View

Could you explain about Unilateral Renal Agenesis?

I have been diagnosed with URA after having a CT scan done. I have no health issues related to my sole kidney (the right one), I eat a healthy diet, drink more water and watch my intake of...

25 November 2019 7,347 2 View

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I am trying to create a FIS fuzzy logic file on Matlab. I am referring myself to a pdf which has the rules and triangle form of the membership. The display range of the function is from - 0.2 to...

09 August 2019 8,016 6 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View