Is there any method to prevent extracellular vesicles from aggregating?

More Carmen María Picazo Córdoba's questions See All

FACS markers to characterize rat bone marrow-derived macrophages?

Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...

15 July 2024 4,615 1 View

Do anyone experiment occasional staining problems with cryoprotectant containing Polyvinyl-pyrrolidone and Ethylene glycole?

Occasionally we observe lack of staining in the inner part of a skin section processed with indirect immunofluorescence. Can be due to incomplete removal of the cryoprotectan during the wash? Any...

07 July 2024 8,200 2 View

Dyslexia and Virtual Reality yes or no?

Do you think the use of virtual reality with dyslexia students is good?

23 June 2024 8,437 2 View

Can anyone recommend bibliography on the analysis of rage from an affect studies' perspective?

I'm analysing a novel in which the said emotion is fundamental to understand the development of characters. Thank you in advance.

22 June 2024 4,859 8 View

If I am doing a thematic analysis based on one group, is it okay to add insights from another group of interviewees?

I am doing a thematic analysis from interviewees done with my main group of interviewees. I also have done two interviews with another group of interviewees which provided interesting insights...

21 June 2024 3,305 6 View

¿En qué medida las tecnologías educativas están siendo efectivas para promover el desarrollo de habilidades para la vida?

las

19 June 2024 2,125 3 View

Is there any way to avoid albumin contamination in isolated extracellular vesicles?

Hi, I am doing western blotting of isolated extracellular vesicles, and I have albumin and calnexin contamination that I don't know why. Is there any way to avoid it?

16 June 2024 9,907 4 View

Is it okay to structure the results section of a thematic analysis based on your research questions?

I am going into my inductive thematic analysis with three research questions. In the results section, is it okay to structure it according to these three research questions, presenting the themes...

16 June 2024 1,116 3 View

How can I add my old article to my profile, if I am the first author, but I have different name before marriage?

Please note, that this is my first manuscript under the name Mária Čápová. Then I have been married, and my name change to Plachá, but I would like to have this article in my profile. Ontogenetic...

16 June 2024 7,178 0 View

Can I design a probe for taqman qpcr in antisense?

I have a target of sequences for primer design for qPCR with taqman. The issue is that when I design a sense probe the probe makes dimers with forward and reverse with a very high probability at...

16 June 2024 1,506 1 View

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

10 July 2024 3,050 5 View

Which is the best small Extracellulare Vesicles (sEVs) concentration to obtain enough RNA for miRNA analysis?

Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...

18 June 2024 2,799 4 View

Is there any way to avoid albumin contamination in isolated extracellular vesicles?

Hi, I am doing western blotting of isolated extracellular vesicles, and I have albumin and calnexin contamination that I don't know why. Is there any way to avoid it?

16 June 2024 9,907 4 View

What are the laboratory precautions taken by the user when handling cancer cells or cancer cell derived extracellular vesicles?

Please give some insight on this

06 June 2024 2,016 2 View

Will ultracentrifugation be replaced as the most widely used method for isolating extracellular vesicles?

Ultracentrifugation is the most widely used method for isolating extracellular vesicles (EVs), despite the significant structural damage that EVs suffer during the process.

16 May 2024 9,432 1 View

Why do people still refer to female ejaculation?

In the younger boy there is no ejaculate because the prostate and seminal vesicles are not yet functionally developed, and in the female those glands are rudimentary and never develop. (Alfred Kinsey)

29 April 2024 6,483 0 View

Why I fail to inhibit extracellular microvesicles?

I tried to inhibit extracellular microvesicles(MVs) secreted from cells, through using Rock inhibitor(Y27632), calpeptin and several siRNAs(siCDC42, siTSG101, siARF1), but failed to decrease MVs....

23 April 2024 7,369 0 View

Estimation of the correct time of cell death after radiotherapy?

Dear Colleagues, We plan to isolate and characterise extracellular vesicles released by dying cells during radiotherapy of head and neck squamous cell carcinoma. Given the duration of...

14 April 2024 494 3 View

Hello, any lentivirus vector to permanently transduction of cells for fluorescent cellular membrane?

I am planning to study extracellular vesicles, and to do this I need cells with a fluorescent membrane (GFP, Tomato, etc.), do you guys have any suggestion of lentivirus vector? Thanks!

02 April 2024 1,944 0 View

Extracellular vesicle characterisation using immunostaining?

Hello dearest EV people! I want to plate my isolated EVs on coated glass coverslips and image them with confocal microscopy for CDs and other EV markers. I wonder if anyone tried a normal IF...

21 March 2024 3,344 1 View