I am looking for any suggestions on how to extract bacterial DNA from stool samples collected and stored in 95% ethanol. (The extracted DNA will be used for 16S rRNA sequencing)

- Kits used?

- How much stool was used in the extraction? How much starting material?

- Does one remove the ethanol before starting the extraction process or is the stool sample homogenized in the ethanol?

I have tried using the PowerFecal / PowerSoil kits (QIAGEN) but I got very little (if any) DNA and the quality was terrible.

Any suggestions / protocols are welcome :)

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