CRISPR-Cas9: Are there any alternatives to the use of fluorescent PCR and capillary electrophoresis?

More Patrícia Gomes Ferreira's questions See All

How can I improve purity of DNA extracted from cyanobacteria using Dynabeads?

I am trying to extract DNA from the cyanobacteria Microcystis aeruginosa using the Dynabeads DNA DIRECT Universal kit for the purpose of on-site detection. Yield is acceptable at ~100-500 ng/uL,...

01 July 2024 10,003 1 View

How do I add more research done?

How can I add additional published work?

03 June 2024 4,045 2 View

Do you know of any questionnaire that can measure the glass ceiling in female engineers and another that can measure their work performance?

Greetings everyone, I am doing a research project on the effect of the glass ceiling on the work performance of women engineers in the construction sector. Do you know of any questionnaire that...

01 June 2024 7,226 0 View

How can I change the coordinate system in Abaqus using Python?

Hello, I'm writing Python scripts for Abaqus and I'm facing a problem. I need to change the coordinate system in a .odb file before extracting data but I'm stuck. I can create my coordinate system...

30 May 2024 7,431 3 View

Z-axis alignment in a protein for z-axis constraints in Amber software?

Good morning, I am running some Steered molecular dynamics in Amber 22 and want to set some restraints in the z-axis so as to imply certain distances from my ligand to its substrate. My goal is...

27 May 2024 7,082 1 View

Novel and future lubricants and additives for hybrid electric vehicles?

nano additives and lubricants for hybrid electric vehicles

24 May 2024 8,363 3 View

Does anyone share his experience regarding the Autophagy activity in plants under drought stress stress?

Please share your ideas and suggestions regarding that "Exogenous application of melatonin reduces autophagy activity in plants under drought stress conditions". If someone knows the phenomenon...

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What tool can predict E. coli protease cleavage sites?

I am observing cleavage in my construct expressed in E. coli and attempting to identify the responsible proteases. Mass spectrometry analysis has revealed the sizes of the fragments, enabling...

12 May 2024 6,540 2 View

Hey, is there anyone who used irradiations for high entropy alloy at room temperature ?

hey, is there anyone who used irradiations for high entropy alloy at room temperature ?

28 April 2024 1,824 1 View

Is there anyone who used iiradiation exp at room temp for high entropy alloy?

is there anyone who used iiradiation exp at room temp for high entropy alloy? can you please give me some suggestion

28 April 2024 2,003 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

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Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

PCR showing no bands - Master mix and primers didn't mix?

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31 July 2024 2,406 6 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Michael Halim Popular answer

Interesting question. Looking forward to the discussion.

Michael Halim

Katie A Burnette

Are you referring to the techniques from this paper?

https://www.pnas.org/content/111/29/E2967

Please clarify your question as you are referring to at least 4 different techniques which all have different uses.