Anyone have any knowledge on AAV tracing?

Is it possible that rAAV vectors (while carrying some fluorescent tags) still cross synapses and pass from one neuron to the other? Any evidence, publication on this issue?

04 May 2014 9,699 8 View

Is there a step-by-step tutorial as to how to conduct patch-clamp experiments?

Patch tutorial

10 November 2013 4,214 12 View

Difficulties working with Calcium Crimson?

We've been trying to run calcium imaging experiments with the red Calcium Crimson and somehow it does not load our neurons well. We are quite successful with Oregon Green BAPTA and Fluo4. These...

10 November 2013 598 4 View

Image analysis to count and measure the diameters and fluorescence intensity of immunofluorescent neuronal somata?

I have fluorescent images with labeled neurons and I need to count, analyze and sort them by size and fluorescence intensity. I have plenty of these images so I don't want to do this manually with...

10 November 2013 5,101 8 View

Does anyone know if microRNA sequences can pass through neuronal gap junctions? I know they do go through gap junctions connecting glioma cells.

Does anyone know if microRNA sequences can pass through neuronal gap junctions? I know they do go through gap junctions connecting glioma cells.

31 December 2012 1,422 1 View

Is there any fluorescent dye or tracer that flows through neuronal gap junctions?

I'd like to inject a visible compound into retinal neurons in an in vitro prep and to monitor its movement from the injected cell into gap junction coupled neighbors. Is there any compound that...

11 December 2012 6,962 26 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View