Extracellular a-Cx36 antibody

Can anyone recommend me an a-Cx36 serum that recognizes the extracellular domain of Cx36? Another requirement is that tha AB has to work in the tissue (not only in cell cultures).

04 May 2017 3,927 5 View

Anyone have any knowledge on AAV tracing?

Is it possible that rAAV vectors (while carrying some fluorescent tags) still cross synapses and pass from one neuron to the other? Any evidence, publication on this issue?

04 May 2014 9,699 8 View

Is there a step-by-step tutorial as to how to conduct patch-clamp experiments?

Patch tutorial

10 November 2013 4,214 12 View

Difficulties working with Calcium Crimson?

We've been trying to run calcium imaging experiments with the red Calcium Crimson and somehow it does not load our neurons well. We are quite successful with Oregon Green BAPTA and Fluo4. These...

10 November 2013 598 4 View

Image analysis to count and measure the diameters and fluorescence intensity of immunofluorescent neuronal somata?

I have fluorescent images with labeled neurons and I need to count, analyze and sort them by size and fluorescence intensity. I have plenty of these images so I don't want to do this manually with...

10 November 2013 5,101 8 View

Is there any fluorescent dye or tracer that flows through neuronal gap junctions?

I'd like to inject a visible compound into retinal neurons in an in vitro prep and to monitor its movement from the injected cell into gap junction coupled neighbors. Is there any compound that...

11 December 2012 6,962 26 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

No title

25 February 2021 5,713 1 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

How can we determine the band gap of a composite material that is made up of a composition of direct and indirect band gap semiconductor materials?

I have a direct band gap material and an indirect band gap material. If I made a composite of both through physical mixing. Then how can I determine the band gap of such a material as the Tauc...

17 February 2021 8,104 4 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Which miRNA arm is a right target for analysis, 3p or 5p?

I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...

16 January 2021 3,792 4 View