Bela Volgyi

7 Questions 18 Answers 0 Followers

Questions related from Bela Volgyi

Extracellular a-Cx36 antibody

Can anyone recommend me an a-Cx36 serum that recognizes the extracellular domain of Cx36? Another requirement is that tha AB has to work in the tissue (not only in cell cultures).

05 May 2017 3,969 5 View

Anyone have any knowledge on AAV tracing?

Is it possible that rAAV vectors (while carrying some fluorescent tags) still cross synapses and pass from one neuron to the other? Any evidence, publication on this issue?

05 May 2014 9,761 8 View

Image analysis to count and measure the diameters and fluorescence intensity of immunofluorescent neuronal somata?

I have fluorescent images with labeled neurons and I need to count, analyze and sort them by size and fluorescence intensity. I have plenty of these images so I don't want to do this manually with...

11 November 2013 5,170 8 View

Difficulties working with Calcium Crimson?

We've been trying to run calcium imaging experiments with the red Calcium Crimson and somehow it does not load our neurons well. We are quite successful with Oregon Green BAPTA and Fluo4. These...

11 November 2013 634 4 View

Is there a step-by-step tutorial as to how to conduct patch-clamp experiments?

Patch tutorial

11 November 2013 4,271 12 View

Does anyone know if microRNA sequences can pass through neuronal gap junctions? I know they do go through gap junctions connecting glioma cells.

01 January 2013 1,496 1 View

Is there any fluorescent dye or tracer that flows through neuronal gap junctions?

I'd like to inject a visible compound into retinal neurons in an in vitro prep and to monitor its movement from the injected cell into gap junction coupled neighbors. Is there any compound that...

12 December 2012 7,015 26 View