what are the precautions that has to be taken care before storing exosome for future miRNA isolation purpose.
What should be the least amount of exosomes (in terms of protein content) that can be used for miRNA isolation
Hello .. I hope this will be helpful:
http://www.journalofextracellularvesicles.net/index.php/jev/article/view/26913
Hi Zaffar,
Most people say its fine to freeze your exosomes at -20C or -80C for extended periods before isolating materials from them. If collecting RNA, I will suspend pellet in Trizol and freeze at -80C if not isolating RNA immediately, and RNA should be stable in Trizol frozen. As for how much exosomes to use, it is highly variable between cell types and I tend to isolate RNA from between 10mL or 20mL conditioned media depending on the yield I typically get from certain cells.
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