Phillip Munson

2 Questions 18 Answers 0 Followers

Questions related from Phillip Munson

What is the best way to add a miRNA spike-in normalization control to my samples if I am starting with already isolated RNA?

I have samples of exosomal miRNA that I want to validate with TaqMan after doing miRNA Array, and I only now have cel-miR-39-3p that I want to add to my samples before doing cDNA synthesis with...

07 July 2016 3,971 10 View

PKH labeling of exosomes: Why do some investigators resuspend their exosome pellet in PBS rather than Diluent C?

Is Diluent C alone, harmful to exosome stability perhaps? Salts present in PBS reduce the staining efficiency of the dye so am curious why people do this step. Just want to clarify, if anyone can...

10 October 2015 6,726 11 View