When stained with DAPI, why does human nuclei gives a diffused pattern while the mouse nuclei gives a punctuate pattern?
Hi Zaffar,
The reason for the punctate appearance of DAPI in mouse nuclei is because of a close association of the peri-centromeric heterochromatin. These regions of heterochromatin are tightly packed, resulting in a high DNA concentration. Thus there is more DAPI in these regions. Additionally DAPI binds to A-T sights, which are highly concentrated in repetitive sequences found in heterochromatin. This is why they appear brighter, and overall less diffuse when compared to human nuclei, in which this close association of peri-centromeric heterochromatin regions is not observed.
In summary then: the punctate pattern is nothing out of the ordinary ;)
Hope this helps!
Thnx.can you refer a paper to me where I can find the Matter in more detail
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