Electroporation of J774.2 murine macrophage cell line

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SPSS categorical variables into index variable?

30 April 2020 2,246 3 View

Hi, I'm doing the alkaline comet assay but I usually find halos around the cells and not comets. Does anyone know why this happens?

My lysis Buffer contains 5M Nacl, 0.5M EDTA, 1M tris ph 10, 10 MNaOH, Then 1% triton and 10% DMSO is added on the day of the experiment. Electrophoresis Buffer contains 0.5M EDTA, 10M NaOH and 1%DMSO

24 September 2019 7,170 1 View

How do I easily dissolve chitin in freshwater invertebrates?

I am doing a syudy on microplast in freshwater and I need a simpel yet effectiv way to dissolve the exoskeleton of the invertebrates I catch without damaging the microplastic

05 September 2018 4,652 4 View

What is the best way to ask study participants about race & ethnicity?

I have been making a question to identify a ethnic group for my PhD research. Could you suggest what the best way to make participants to feel like answering in a questionnaire to categorize the...

24 May 2015 6,703 8 View

How to make a preparation of mouse liver to be able to detect a protein in the plasma membrane of hepatocytes by western blotting?

The primary antibody that I use to detect my protein of interest by western blotting is not very sensitive, so I think I will need some kind of enrichment for membranes in my prep. My protein of...

26 February 2013 7,876 3 View

Could you let me know if I can use Factor analysis for ranking questions 1-3?

When I tried to make a factor analysis for Internet survey for the ranking items (questions) 1-3 on SPSS version 22, the following warning message came up and I cannot carry on (I also attach the...

01 January 1970 3,467 2 View

Sidman, J. D. (1995). The team approach to cleft and craniofacial disorders. Cleft Palate–Craniofacial Journal, 32(5), 362.

Hi Does anyone have this article or know where I can find it? Sidman, J. D. (1995). The team approach to cleft and craniofacial disorders—The downside. Cleft Palate–Craniofacial Journal, 32(5),...

01 January 1970 7,860 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View