SPSS categorical variables into index variable?

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30 April 2020 2,119 3 View

Hi, I'm doing the alkaline comet assay but I usually find halos around the cells and not comets. Does anyone know why this happens?

My lysis Buffer contains 5M Nacl, 0.5M EDTA, 1M tris ph 10, 10 MNaOH, Then 1% triton and 10% DMSO is added on the day of the experiment. Electrophoresis Buffer contains 0.5M EDTA, 10M NaOH and 1%DMSO

24 September 2019 7,075 1 View

What is the best way to ask study participants about race & ethnicity?

24 May 2015 6,584 8 View

How to make a preparation of mouse liver to be able to detect a protein in the plasma membrane of hepatocytes by western blotting?

The primary antibody that I use to detect my protein of interest by western blotting is not very sensitive, so I think I will need some kind of enrichment for membranes in my prep. My protein of...

26 February 2013 7,790 3 View

Electroporation of J774.2 murine macrophage cell line

Does anyone have experience with transfection (electroporation) of the murine macrophage cell line J774.2? I am planning to use the Neon Transfection System (Invitrogen), and I would be...

02 August 2012 8,697 2 View

Could you let me know if I can use Factor analysis for ranking questions 1-3?

When I tried to make a factor analysis for Internet survey for the ranking items (questions) 1-3 on SPSS version 22, the following warning message came up and I cannot carry on (I also attach the...

01 January 1970 3,396 2 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

16 February 2021 5,101 2 View

How to remove the species data from rda data frame in R (only remain the sites/samples point)?

Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...

15 February 2021 8,982 2 View

How to find statistical data of EEG signals?

Hi all, I hope everyone is doing good. I am working on Machine Learning, I am working on EEG data for which I have to extract statistical features of the data. Using mne library I have extracted...

29 January 2021 6,686 1 View

How can I solve this problem with agarose gel DNA purification of bigger (1-4kb) fragments ?

Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...

24 January 2021 2,190 5 View

Is PV array not available in MATLAb 2020a simulink?

I have MATLAB 2020a. PV array is not present in the simulink library. Can anyone please explain if the name of module has been changed or is it not available anymore? Is there any way to add PV...

24 January 2021 7,962 5 View

How can I transform 50 kb plasmid into ecoli?

I must put 50 kb plasmid into ecoli strain. I think 50 kb is too big for heat shock with ecoli. Is that correct? Will electroporation work? Or do I need to use cos sites and lambda phage...

22 January 2021 8,612 3 View

No title

13 January 2021 2,487 1 View

IET Digital Library Access?

Hello to everyone, I do not have access to the IET Digital Library at the institution where I work. Can those who have access to this library send me the publication whose DOI is given below?...

13 January 2021 9,385 2 View