Mie Rostved Bechshøft

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Questions related from Mie Rostved Bechshøft

How to make a preparation of mouse liver to be able to detect a protein in the plasma membrane of hepatocytes by western blotting?

The primary antibody that I use to detect my protein of interest by western blotting is not very sensitive, so I think I will need some kind of enrichment for membranes in my prep. My protein of...

27 February 2013 7,836 3 View

Electroporation of J774.2 murine macrophage cell line

Does anyone have experience with transfection (electroporation) of the murine macrophage cell line J774.2? I am planning to use the Neon Transfection System (Invitrogen), and I would be...

03 August 2012 8,770 2 View