In my experiment, I utilized a combination of Poloxamer 188 (Kolliphor) and Gibco Anticlumping agent in Dynamis media at a concentration of 0.1% per liter for CHO S cell culture. Surprisingly, I observed that cell growth was inhibited, and the cells proliferated at a much slower rate compared to the control group that did not contain Poloxamer 188 but included Gibco Anticlumping agent. Typically, the cells would enter the exponential growth phase by day 2 (~2.3x10^6), but with the addition of kolliphor Poloxamer 188, it took approximately 4 days for the cell count to increase ( ~1.8x10^6), albeit with 85% cell viability. I am curious to know if anyone else has experienced similar results with this combination of additives in CHO S cell cultures. Can anyone help me in justifying this phenomena.
* The experiment was carried out 4 times so there is no point of error.
Hello Ashwin Naidu
Poloxamer is a component in cell culture medium that is widely used in industrial protein production. It is added to a cell culture medium to enhance the viability of cultured cells. One of its many functions is to act as a surfactant, reducing the force of attachment between the cells and gas bubbles in the cell culture medium and protecting the cells from damage when the bubbles burst. So, in a way it is beneficial in culture.
But unfortunately, significant lot-to-lot variability in poloxamer performance has been observed. When used in a cell culture medium, poorly performing lots of poloxamer can cause reduced cell viability and cell growth rate. Reduced cell viability leads to reduced protein production.
Therefore, there is a need for a simple, inexpensive solution to reduce poloxamer variability and to improve poloxamer performance, particularly for poorly performing lots.
You may want to refer to the link provided below for more information.
https://patentimages.storage.googleapis.com/20/44/88/7d77e5d96a99b0/EP3778702A1.pdf
Best.
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