During splicing, if the junction sequence between exon and intron is found inside an exon, then, whether the spliceosome cleave that exon or not?

More Anaswar S R's questions See All

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

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How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

I am trying to obtain microstructure for Mg-Zn-Sn alloy?

Any suggestions with respect to etchant composition and holding time?

27 July 2024 6,925 2 View

Flow through curved domains?

Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...

25 July 2024 9,058 4 View

What is the Principle of Cysteine-carbazole-sulfuric acid reaction with glucose?

I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...

23 July 2024 3,891 0 View

How to get Scopus Author Index ??

18 July 2024 4,080 3 View

Dear researchers. pl help how to plot jablonski energy level graph and magnetic hysteresis curve in origin?

through origin software

17 July 2024 4,991 0 View

Reversed flow at outlet due to the release of DFBI?

Hi everyone, I am working on a simulation involving restricted canal with ship using DFBI. I am facing reversed flow in my outlet boundaries as the DFBI is released (In 1.25s). Is there any...

17 July 2024 7,032 1 View

Do software tools exist to assess the economic and technical practicality of introducing new food products, such as yogurt with modified starch?

This question explores the world of food innovation and asks if there are computer programs that can analyze the financial and technical feasibility of introducing new food products. For instance,...

13 July 2024 7,446 0 View

Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

How to solve Bubble Error in fiber splicing?

I'm having a bubbling error while splicing 100/350 um optical fiber (core/cladding) on the Fujikura FSM100P+. I have tried some ways such as changing Prefuse power and Prefuse time but to no...

03 July 2024 7,463 6 View

Could you please tell me how to understand this table and above mentioned exons figure?

I am getting problem to exactly identify the SNP position.

18 June 2024 8,184 2 View

Problems with designing syber-green primers for lnc_RNA GAS5?

Hi all, you know this lncRNA has 12 exons and 50 isoforms that just have similar sequences in exon 12. Coud someone recommend me primers for this exon with no need of probes?

15 June 2024 8,055 0 View

How to design RHCE allele primers?

The RH blood group system is encoded by RHD and RHCE genes, and the difference of G>C at the 676 base of exon 5 of RHCE gene represents the RHE and RHe alleles. If you want to design primers...

21 May 2024 7,667 1 View

APOe isoforms Sanger sequencing - good primers and protocol?

Hello everybody. For my project i need to identify the APOe isoforms present on my patient derived lines. My idea was to extract DNA, amplify with PCR and send it for Sanger sequencing -...

24 April 2024 6,359 4 View

Why exon number change by zooming out in Genome Browser?

Why does the number of exons of PTPRQ change by zooming out in Genome Browser site? the NM number is: NM_001145026.2

21 April 2024 8,336 1 View

Can I get a gene expression result in qPCR if the forward primer binds to the exon junction but the reverse primer binds to the UTR?

That gene has four transcripts. To experiment with gene expression, I have designed a qPCR primer from NCBI. But it gives me primer pairs, of which forward primer binds to the exon-exon junction...

16 April 2024 6,510 2 View

Can intron 1 site be used for CRSIPR Cas9 mediated Knock in?

hey, I am designing gRNA target site to check the endogenous expression of protein in zebrafish. For this I am using chop-chop site. But most of the sites are in exonic region. I couldn't find...

13 April 2024 3,757 3 View

CRISPR targeting in cell line, flow or single clone?

I am doing a CRISPR targeting to knock out one intron of my gene of interest in a cancer cell line and replace that intron with GFP. I am wondering if I should pick single cell clone with possible...

17 March 2024 9,713 1 View

Zhiyu Chen

You can calculate its ATGC/CGTA distribution, email me, and I'll give you the method and the program, and three years ago, I entered a biometric contest, and the competition was to identify different bacteria

my email: [email protected]

Katie A S Burnette

I'd recommend you start by reading a molecular biology text book and/or a few review articles on the subject.

Stuart Stephen

Transcript isoforms can contain isoform specific splice junctions - in some instances, individual exons and introns (yes, you can have retained introns in the mRNA) in the transcript may be shorter/longer than their canonical representations. And the forgoing isn't even considering isoform differences in 5' and 3' UTRs, the possibility of alternative TSSs and transcriptional RNA-editing in both pre-mRNA and mRNA.