I've been treating my MDA PCa-2b cells with a component that is known for creating apoptosis, but more interestingly this component is decreasing the expression of intracellular protein beta-arrestin 2, at this time we are trying to figure out the pathway behind it. I am currently looking at ERK; I've been treating my cells (that are already treated with the component) with Interluekin 8 (at difference concentrations) to see the affect of ERK, however I am not seeing much of a difference in the expression for ERK for both my treated cells and control cells. I'm trying to determine why I am not seeing a difference in ERK expression (especially in control cells) when treated with IL-8, possibly MDA-Pca 2b does not express in the CXCR1 and CXCR2 that is associated with IL-8. Any thoughts?
The levels of cxcr1/2 on prostate epithelial cells can change quite a lot depending on the way you grow these cells, what growth factors or hormones they were exposed to, etc. The attached paper should give you some ideas for how to proceed.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1602414/
Hi Tonya,
I have trying to culture the same cells for a while now. But these seem to grow in clumps and not expand at all. I am using the HPC1 media (20 FBS). I wonder if this media works fine with these. What media do you use for your experiments?
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