This may be an amateurish question, but it is a minor point outside my expertise that I want clarification. As an example, cytochrome P450 epoxygenases are often found in microsomes of vascular endothelial cells. Does this mean they are present in the ER of the intact endothelial cell?
Hello. When you isolate microsomes, depending on the method, you may have several organelles. For example, it is easy to co-fractionate mitochondria and ER. You should always use bonafide organelle markers to learn the identity of your microsomes. Depending on the desired separation, whatever you get may be fine as long as you don't overstate your results. Hope this helps.
Giuseppe, as interesting as your answer is, I think you may have clicked on the wrong question.
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