David C. Ellinsworth

9 Questions 119 Answers 0 Followers

Questions related from David C. Ellinsworth

Is there now a consensus that shear stress increases between large and small arteries/microcirculation?

The view that shear is constant across the circulation appears to be dwindling, but is the view that shear increases in small arteries/microcirculation still controversial, or is there now a...

02 July 2016 1,208 6 View

Why do standard buffers, such as Krebs, Holmans etc, have such high glucose concentrations?

Several standard buffer recipes have 11 mM glucose, and also sucrose. I realise buffers need osmolarity, but why such high sugar concentrations? I used to use Holmans in myograph experiments, and...

26 May 2016 8,578 3 View

How do blood vessels restore pressure following autoregulation of blood flow?

In many vascular beds a drop in pressure triggers vasodilation to autoregulate tissue blood flow. But, despite an increase in flow, would this not lead to further pressure drop, and how is the...

26 May 2016 5,502 9 View

Endothelium-derived hyperpolarization (EDH) and endothelium-derived hyperpolarizing factor (EDHF): a general, non-specific debate?

Having recently answered a couple of threads pertaining to EDH and EDHFs, it is apparent that a generalized discussion of the topic on researchgate may be overdue. Generally defined as the...

09 May 2016 136 0 View

In studies using myography does iberiotoxin in itself actually reflect EDHF-type activity?

Studies of NO-independent, hyperpolarization-mediated relaxation using myography often use the selective BKCa blocker iberiotoxin to delineate the role of transferable EDHFs, such as EETs or H2O2....

16 January 2016 2,174 17 View

Has anyone observed patterns in Nox (NADPH oxidase) localization in specific vascular regions?

I'm specifically interested in whether anyone has observed differential expression of Nox1, 2, 4 and/or 5 in the endothelium at regions of disturbed shear, I.e at vessel branchpoints and...

13 January 2016 978 2 View

Does the presence of a protein in microsomes tell you that it is present in ER of the intact cell?

This may be an amateurish question, but it is a minor point outside my expertise that I want clarification. As an example, cytochrome P450 epoxygenases are often found in microsomes of vascular...

28 December 2015 5,222 3 View

What is the subcellular localization of CYP epoxygenases (2C and 2J) in vascular endothelial cells?

I know they are found in microsomal fractions of endothelial cells, suggestive of an ER distribution, but why has their subcellular distribution not been visualized, e.g. IHC and confocal, in...

15 April 2015 4,335 2 View

Has anyone observed an ability of catalase to attenuate responses to NO donors?

Apparently catalase can bind NO, but every other publication I've seen where this protocol has been used (usually as a negative control for endothelial dysfunction studies) shows negative results,...

01 April 2015 218 5 View