13 Questions 146 Answers 0 Followers
Questions related from David C. Ellinsworth
The view that shear is constant across the circulation appears to be dwindling, but is the view that shear increases in small arteries/microcirculation still controversial, or is there now a...
02 July 2016 1,246 6 View
Several standard buffer recipes have 11 mM glucose, and also sucrose. I realise buffers need osmolarity, but why such high sugar concentrations? I used to use Holmans in myograph experiments, and...
26 May 2016 8,624 3 View
In many vascular beds a drop in pressure triggers vasodilation to autoregulate tissue blood flow. But, despite an increase in flow, would this not lead to further pressure drop, and how is the...
26 May 2016 5,547 9 View
Having recently answered a couple of threads pertaining to EDH and EDHFs, it is apparent that a generalized discussion of the topic on researchgate may be overdue. Generally defined as the...
09 May 2016 167 0 View
These are commonly used "specific" blockers of small conductance KCa (KCa2.1-2.3; SKCa; SK1-3), intermediate conductance KCa (KCa3.1; IKCa; IK1) and large conductance KCa (KCa1.1, BKCa, BK1)...
28 April 2016 9,476 2 View
Studies of NO-independent, hyperpolarization-mediated relaxation using myography often use the selective BKCa blocker iberiotoxin to delineate the role of transferable EDHFs, such as EETs or H2O2....
16 January 2016 2,214 17 View
Plasmalemma? ER? I'm interested in information from isolated or cultured cells, but preferably intact arterial tissue.
14 January 2016 8,359 2 View
I'm specifically interested in whether anyone has observed differential expression of Nox1, 2, 4 and/or 5 in the endothelium at regions of disturbed shear, I.e at vessel branchpoints and...
13 January 2016 1,007 2 View
The activation of certain TRP channels, be it directly (for TRPV4) or indirectly (for TRPCs), by EETs is established, but is anyone aware of any others? Maybe someone has preliminary data showing...
05 January 2016 9,576 2 View
This may be an amateurish question, but it is a minor point outside my expertise that I want clarification. As an example, cytochrome P450 epoxygenases are often found in microsomes of vascular...
28 December 2015 5,262 3 View
I am wondering if anybody is aware of studies that have looked at internal elastic lamina fenestrations and trans-IEL endothelial/smooth muscle projections extending through these fenestrations? I...
29 April 2015 4,877 3 View
I know they are found in microsomal fractions of endothelial cells, suggestive of an ER distribution, but why has their subcellular distribution not been visualized, e.g. IHC and confocal, in...
15 April 2015 4,361 2 View
Apparently catalase can bind NO, but every other publication I've seen where this protocol has been used (usually as a negative control for endothelial dysfunction studies) shows negative results,...
01 April 2015 254 5 View
