Why DMEM color is changed?

Why the pink color will change to yellow, when the DMEM media is frozen? however after dissolution in room temperature, media color will change back into pink.

26 October 2020 708 2 View

A genome wide CRISPR/Cas9 screen for drug resistance in cancer cells?

03 November 2019 7,662 2 View

What kinds of single indicators are appropriate, when we calculate multifunctionality?

A (wide accepted) view is that, when we try to address on the biodiversity-ecosystem function relationships, the multifunctionality index should be calculated by a set of activity indicators,...

02 September 2019 8,370 2 View

How to choose solvent C18 column for separating polar compounds?

I have one polar product with an impurity, which has the similar structure and polarity. I used the Hexane:Ethyl acetate:Methanol in 6:3:1with silical gel. And the difference of Rf is around 0.08,...

06 August 2019 3,572 2 View

How to fabricate/make silica spin column for DNA/RNA extraction?

Dear Friends, Does anyone know how to fabricate/make silica spin column for DNA/RNA extraction? Any books, references and videos are welcome. Thanks in advance. GH Yue

27 June 2019 2,456 3 View

How to present the complete results of stepwise regression?

The common form is the presentation of regression equation, with the coefficients of determination and p value included in the brackets. But we know that the beta coefficients and sum of squares...

02 March 2019 6,064 6 View

Do you have information of extremely stable DNA?

Dear friends, I am looking some extremely stable DNA sequences. If you have some info on this, please let me know. Regards, Gen Hua

24 June 2018 591 2 View

Does anyone know if there is a method for the complex CRISPR/dCas9 to become fluorescent only when it binds to the gDNA?

Hi, I saw that it is possible to target and image a single locus on the genomic DNA with a cocktail of CRISPR/dCas9 following the CasFISH method. However, the signal is quite diffuse and I want to...

15 June 2018 1,583 2 View

Can RNA shuttle between the nucleus and cytoplasm?

Hi, everyone As we all know, a protein can shuttle between the nucleus and the cytoplasm. Whereas many proteins are selectively transported from the cytoplasm into the nucleus, most RNAs are...

10 April 2018 9,318 3 View

What is the distribution of most frequently outcome when sampling from a multinomial distribution ?

08 April 2018 1,890 5 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View