4 Questions 131 Answers 0 Followers
Questions related from Sharad Purohit
I am trying to purify IgM from precipitate obtained from serum after dialysis against 10mM sodium acetate buffer pH 5.6. Will a 70 ml Bed volumn column will do the necessary separation to remove...
05 May 2015 7,690 5 View
I would like to clone a cytokine in pet32 vector. The gene sequence was downloaded from NCBI and I checked the coding region for protein. How do I make sure that the gene is in alignment with the...
09 September 2013 8,999 2 View
Hello, what is the percentage composition of CD3+ cell CD4+ cells CD80+ cells CD86+ cells CD11c+ cells in spleen of a mouse
07 July 2013 6,846 0 View
I would like to isolate the IP products using human serum as a source of antibodies. Several papers report the use of sepharose 6b columns to fractionate the IP products. I am looking for a quick...
03 March 2013 2,935 2 View