Please i need a proper protocol to isolate intramuscular preadipocyte from the rabbit
Hi I am having issue's with Gibson assembly, seeing as I am new in the filed of cloning, expert feedback would be very much appreciated I am trying to clone two fragments (frag 1 [185bp, 145bp w/o...
29 January 2021 1,406 2 View
Alternatively I would like to know if there's any bacterial promoters that are recommended for expressing small RNA molecules?
19 February 2020 1,101 4 View
Needed for a research project
03 August 2018 446 3 View
The techniques of separation of chlorophyll from methanolic plant extracts.
20 May 2015 8,064 4 View
Has anyone worked on the isotherm and kinetic studies of Pb2+ removal with zerovalent iron nanoparticles?
25 August 2014 8,147 3 View
I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit
20 February 2021 3,366 5 View
I want to find a database(s) or a method where I can find and bulk download all the rabbit MHC genes and domain annotation. In humans using Ensembl, all MHC genes have names and domains but in...
28 January 2021 7,722 2 View
I know this has been asked before, I repropose the question hoping to get an up to date fedeback. I have already tested the E-8 and 6-2H-2F from Santa Cruz with really poor results. Unfortunately...
19 January 2021 6,270 2 View
I have generated two antibodies against two peptide fractions of a protein in the rabbit. I want to use these two antibodies (Ab-1 and Ab-2) in indirect sandwich ELISA for the detection of...
11 January 2021 1,754 4 View
I just realized that I incubated my sections with two primary antibodies both of which are raised in mouse. I don't want these sections to go to waste and am hoping to wash away any bound primary...
18 November 2020 3,965 3 View
I have 3 groups of animal apart from PBS controls where i treat them under different immunosuppressive conditions. when the animals are subjected to partial or no immunosuppressive conditions, i...
31 August 2020 9,012 5 View
In IHC for double staining i use DAB and AP. DAB works fine but AP after mounting is a headache as i see precipitates and also sometimes dissolves.I have tried drying the slides a bit and then...
26 July 2020 219 2 View
Its study for alloxan-induced diabetes in the rabbit.
14 July 2020 2,869 1 View
I would like to do a double immunofluorescence staining TOM20 and LC3 but both primary antibodies is produced in rabbit. Can anyone help me with a protocol?
28 June 2020 9,412 12 View
I am working with rabbit spleen and as it is difficult to obtain antibodies against rabbits as most of them are raised in rabbits. I had to purchase a few antibodies raised in rabbits. But as...
17 June 2020 3,930 8 View