Please i need a proper protocol to isolate intramuscular preadipocyte from the rabbit
my research is focused on IMF of rabbit. however, unlike other livestock like beef cattle and pork, it is difficult to see IMF deposition on the longissmus muscle of rabbits. please i need a...
29 January 2024 2,157 0 View
I am currently on my PhD research, which focused on validating some identified long non-coding RNAs that regulates intramuscular fat deposition in rabbit, but I lack the basic experimental skills...
17 January 2024 8,020 2 View
If a video is available, it will assist me more. And the adipocyte tissue culture procedures
25 September 2023 4,632 0 View
Is Physiotherapy intervention really needed during the acute phase of Neurosyphilis? If yes, what is the best management approach (evidence-based)?
10 September 2022 8,022 2 View
To enable us generate place records for Nigeria and Africa for early response in the fight against NCDs and Chronic disease. To show that Geographic Information matters in disease control.
13 July 2021 6,875 0 View
Hi From experience does anyone know if there is a link between cell passage number and gene-editing efficiency? I suspect there might be an inverse relationship (ie high passage number = lower...
04 July 2021 4,771 2 View
Hi I am having issue's with Gibson assembly, seeing as I am new in the filed of cloning, expert feedback would be very much appreciated I am trying to clone two fragments (frag 1 [185bp, 145bp w/o...
29 January 2021 1,620 2 View
I mean, a study plan that draws up a preliminary timetable for completing PhD research studies. It could be in an Excel sheet or MS-word format.
14 December 2020 1,996 3 View
I would like to know if T7 in vitro transcription can be effectively terminated by pol II terminators (specifically bGH poly (A) signal)? And in reverse can a pol II promoter (eg CMV promoter)...
08 June 2020 9,595 0 View
Alternatively I would like to know if there's any bacterial promoters that are recommended for expressing small RNA molecules?
19 February 2020 1,246 4 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Hi everyone, I have found myself in the rabbit hole of attempting to identify a robust method of conducting a narrative synthesis for my protocol. I am reading a variety of methods in both design...
17 July 2024 8,082 3 View
Is there same type DNA and RNA testing. Why not rabbit max?
15 July 2024 3,001 2 View
Greetings all, My group has been trying to reproduce 3t3-L1 cell (preadipocytes) differentiation. The typical protocol involves 3 steps: the first step and second step both last for 2 days, and...
18 June 2024 2,728 1 View
I have a peculiar problem - in FBS or albumin containing media my cells (preadipocytes) are very easy to image (and therefore quantify using a combination of ilastik and CellProfiler). However,...
28 May 2024 3,780 0 View
The development of mRNA vaccines on mice and rabbits?
18 May 2024 7,475 2 View
This research question delves into the intriguing relationship between the spread of myxoma virus and various ecological factors influencing wild rabbit populations. By exploring how climate,...
15 April 2024 3,600 1 View
Hi all, I have been attempting to blot for LC3I and II and i have been having a strange issue, the bands seem to be showing up around 23 kDa. I have done the blot twice now and still the same...
28 March 2024 3,621 5 View
I induced the preadipocyte after they reached 100% confluency. Then, I treated them with differentiation medium (hg DMEM 10% FBS, 1X P/S, IBMX 0.5mM, Dex 1uM, and Insulin 10 ug/ml) for 2 days....
18 February 2024 8,219 0 View
Actually i work on visceral and subcutaniuse fat from mice and culture them with both methodes of 2d and 3d. My cells are not differentiated well. I need the exact protocol to compair with mine.
14 February 2024 6,752 0 View