Does COCl2 needs to be prepared fresh everytime to induce hypoxia in vitro? Or you can simply store a stock solution at a certain temperature?

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Niroj Kumar Sethy

Dear Prasad,

You have to prepare flash solutions of cobalt chloride. Since it is a weak Lewis acid, fresh solutions for both in vitro and in vivo applications is recommended.

Pooja Gupta-Saraf

I just used the one stock solution stored at room temperature and did not face any trouble inducing HIf1a stabilization. I only worked in-vitro so I can not say about animal work.