Prasad Sulkshane

5 Questions 4 Answers 0 Followers

Questions related from Prasad Sulkshane

Problem with thiol-induced cleavage of target protein from Intein-CBD tag???

I have successfully expressed and purified target protein using pTXB1 vector from NEB. We confirmed the desired molecular weight by SDS PAGE and identity of the protein by mass spectrometry. To...

13 May 2020 8,793 1 View

Can I use lentiviral packaging plasmids (pMD2.G and pPAX2) with a retroviral target plasmid?

Will the viral packaging work with a combination of a retroviral target plasmid and lentiviral packaging plasmids (pMD2.G and pPAX2)? Although I saw Syncytia formation in the HEK293FT cells post...

07 January 2019 6,070 4 View

What is the knockout efficiency of pSp-Cas9(BB)2A-Puro system?

I am using the pSp-Cas9(BB)2A-Puro system for creating a knockout cell line. My question is after the drug selection of the transfected cells, is it advisable to pool together all the colonies or...

02 October 2018 9,709 3 View

Can I substitute OptiPrep for Percoll for isolation of mitochondria from mammalian cells?

Hi...I am trying to isolate mitochondria from cultured mammalian cells using OptiPrep density gradient. However, in the literature I found that Percoll has been widely used with the best...

19 October 2017 1,334 7 View

Does COCl2 needs to be prepared fresh everytime to induce hypoxia in vitro? Or you can simply store a stock solution at a certain temperature?

COCl2-induced hypoxia in vitro

31 July 2017 7,431 2 View