I am working on an in-vitro model of hypoxia-reoxygenation (H/R) in cardiac cells (H9c2). Due to the unavailability of a hypoxic incubator, I am exploring alternative methods to create hypoxic conditions.

The ischemic buffer I am using contains the following constituents: 1 mM NaH2PO4, 24 mM NaHCO3, 2.5 mM CaCl2, 1.2 mM MgCl2, 118 mM NaCl, 16 mM KCl, 20 mM Sodium-L-lactate, 10 mM 2-Deoxy-D-glucose

My question is to what extent can this ischemic buffer alone replicate the key features of hypoxia observed in a hypoxic incubator setting within the context of an in vitro H/R model for cardiac cells?