100 ppm means 1 part per million of water by weight. 1 liter of water weighs 1 kilogram. One-millionth of a kg is 1 mg. So 100 ppm is 100 mg of dye per liter of water.
To prepare a 100 ppm (parts per million) methylene blue stock solution of 1 liter, you need to calculate the amount of methylene blue dye required. Here is the step-by-step calculation and procedure:
Calculation
1 ppm means 1 milligram of solute in 1 liter of solution. 100 ppm means 100 milligrams of solute in 1 liter of solution.
Therefore, for a 100 ppm solution:
100 ppm=100 mg/L
Required Weight
Since you need a 100 ppm solution in 1 liter of water, you need: 100 mg= 0.1g
1) Weigh the Dye: Accurately weigh out 0.1 grams (100 milligrams) of methylene blue dye using an analytical balance.
2) Dissolve the Dye: Add the weighed dye to a volumetric flask or a suitable container.
3) Add Water: Add distilled or deionized water to the container to make up the total volume to exactly 1 liter.
4) Mix the Solution: Stir or shake the container thoroughly to ensure the methylene blue dye is completely dissolved.
To prepare a 100 ppm (parts per million) methylene blue stock solution in 1 liter of water, you need to know the molecular weight of methylene blue and apply the definition of ppm.
Definition and Calculation
- 1 ppm means 1 mg of solute per liter of solution.
- Therefore, 100 ppm means 100 mg of solute per liter of solution.
Given:
- Desired concentration: 100 ppm
- Volume of solution: 1 liter
Steps to Calculate the Required Weight
1. Determine the amount of methylene blue needed:
- Since 100 ppm is 100 mg/L, you need 100 mg of methylene blue per liter.
2. Convert mg to grams:
100mg = 0.1g
Weighing the Dye
Weigh out 0.1 grams of methylene blue dye accurately using an analytical balance.
Preparing the Solution
1. Weigh the dye:
- Use an analytical balance to weigh 0.1 grams of methylene blue dye.
2. Dissolve the dye:
- Add the weighed dye to a 1-liter volumetric flask.
3. Add water:
- Fill the flask with distilled or deionized water up to the 1-liter mark.
4. Mix thoroughly:
- Stir or shake the solution until the dye is completely dissolved.
To make a 100 ppm methylene blue stock solution in 1 liter of water, weigh out 0.1 grams of methylene blue dye and dissolve it in 1 liter of distilled or deionized water.
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