From my understanding biotinylate hyaluronic acid binding protein (bHABP) binds to the link protein between HA and proteoglycan monomers. Would recombinant HA have this link protein and would bHABP bind to HA in a synthetic matrix?
We have a project for school, where we have to do a derivatisation of glucose. Here we add glucose in demi water with aniline and HCl, after variations in time and temperature, we still don't get...
19 December 2023 6,323 0 View
What is the negative control, the positive control and the reagent control in the agarose gel electrophoresis that is prepared for the identification of PCR products? Could a ladder of DNA be a...
01 December 2023 405 8 View
Hi, I am new here. I'm a PgDip Psychology student from the University of Derby (Online) and I also work as a Research Practitioner for Cornwall Partnership NHS Foundation Trust. I am currently...
09 November 2023 1,217 0 View
We have used a Novus kit but the results were disappointing.
10 July 2023 9,897 4 View
Hello! I have received 10 mg of SB242084 hydrochloride (https://www.sigmaaldrich.com/CA/en/product/mm/506417). Since the sigma datasheet said it would be water soluable, I tried to dissolve it...
20 April 2023 7,871 1 View
What is the meaning of the string (*) in CYP3A4*22, knowing that this SNP has the ID rs35599367?
13 October 2022 3,405 3 View
I am using Autodock Smina - a fork of Vina to perform docking. I have docked the ligands to my receptor (including decoy). However I am having trouble combining them into a SDF that keeps the...
23 March 2022 3,512 1 View
Hi, I have data from a toxicology study where I see a hormesis trend in the data and I want to make sure my hormesis model is appropriate. My model is driven by one value in the highest treatment...
14 March 2022 1,430 1 View
I was wondering if anyone can tell me where to find the Verbalizer-Visualizer Questionnaire (VVQ)... I'm researching the relationship between visual perception and cognitive styles, and I'd like...
20 December 2021 9,340 4 View
I have the reference SNP (rs) of some SNPs, how can I search to know the exact location of these SNPs on the gene? For example what is the exact location of the SNP with ID rs17716499? does it...
10 September 2021 6,295 4 View
I’m doing cell-surface biotinylation with HEK293 cells and after all the washing and biotin incubation steps (washing with PBS ph8, neutralizing unreacted biotin with 40mM Tris ph8) many of my...
23 July 2024 4,740 3 View
I have noticed that, regardless of whether isolating primary microglia from humans or mice, purity is generally assessed using CD11c flow cytometry staining. Why not use CD11b instead?
02 July 2024 9,411 2 View
Usually, I use cDNA and perform gradient PCR for primer standardization. What kind of sample cDNA do I have to use in this case and what steps do I need to consider for standardization?
26 May 2024 8,124 0 View
Hi All, Our group is doing some flow cytometry assays using an HLA-A*0201 tetramer presenting an HBV peptide (FLLTRILTI) and we're getting some strange results... We ran tetramer flow on a bunch...
16 May 2024 8,123 1 View
In My project, I want to detection of xx (not important) antigen spesific t cell. I will use peptivator (Militenyl) and then detection with florospot assay. However, ı want to detection antigen...
19 March 2024 2,054 0 View
Hello! I am trying to test the action of a drug that activates T cells in vitro and I want to determine whether it can do this in the face of inhibition from either the PD-1 or CTLA-4 signaling...
05 March 2024 2,878 0 View
I am developing a protocol for the generation of antibodies using the Phage display method. Could anyone share a protocol for bio panning of biotinylated antigens?
05 March 2024 9,549 3 View
Hi all, I would appreciate any help with the following question: I have planning to have an Alzheimer's Disease rat brain tissue that I am looking planning to look for markers for amyloid-beta,...
21 February 2024 5,140 2 View
Looking to perform a non-radioactive EMSA to study protein binding to ssDNA. Before doing so, I’m looking to optimize detection. The two detection series are: 1) biotinylated oligo, strep-HRP...
01 February 2024 8,161 0 View
Oligo length is 19bp
10 January 2024 4,299 1 View