Looking for appropriate statistical test for Likert scale with 5 domains (sub-sets) to see if any correlation?

Cohort = 19 so quite small One domain would be DV and other 4 x IV's I have access to SPSS and excel. I'm going round in circles trying to find the best fit. I would use multiple regression but...

31 March 2020 2,640 4 View

How to register in EMBL gene bank?

Hi Dear colleagues, i had registered 3 DNA fragments in NCBI through DDBJ gene bank , i understand that the two gene banks in addition to EMBL gene bank are linked and the data is interchanged...

21 July 2018 7,324 1 View

What is the benefit of registering a DNA sequence in NCBI?

Many researchers tend to register the DNA sequence that they work on in NCBI or DDBJ or EMBL. What is the benefit of the registration for the researcher and for in general and what is the...

19 July 2018 5,122 3 View

How to isolate TaqI endonuclease from E-coli ?

in the synthesis of TaqI endonuclease using Ecoli strain by NEB company for example, where from they get the gene coded for this enzyme, what is the function of this enzyme in the original...

07 July 2018 7,944 3 View

Is there a difference between publishing a research article as a book chapter and/or in a peer-review journal

03 July 2017 213 19 View

What is the SPSS equivalent of _N in Stata?

I am trying to write the syntax to calculate the median number of visits per facility in a data set where each row/case is one visit. In Stata I would do: bysort providercode: gen littlen=_n...

04 December 2016 4,985 2 View

Favorite program/app to organize digital images into a searchable/filterable database??

08 June 2016 3,850 2 View

Two-way ANOVA, how can independent variable be significant (p=0.1) in test of between subjects and insignificant difference in pairwise comparison?

Dear all, I am running a two way ANOVA with two dependent variables (domestic/cross-border (dummy variable) and industry (five categories)) on one dependent variable (performance, measured on a...

28 May 2016 3,540 3 View

Which statistical tool to use if I have 5 independent variables and one dependent variable and 5 different groups?

Dear all, I want to study what the effects of 5 independent variables are on 1 dependent variable. I have succeeded in this but now I want to study if the relationship of the independent variable...

25 April 2016 9,347 4 View

Is there a cell line available that is positive for insulin?

From primary cells cultures of the human pancreas, we are getting positive immunostaining of cells for anti-insulin and anti-glucagon. We also get positive results from RT-PCR using a primer for...

06 March 2013 2,572 17 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View