Hello!
I am trying to test the action of a drug that activates T cells in vitro and I want to determine whether it can do this in the face of inhibition from either the PD-1 or CTLA-4 signaling pathways.
Is there a good agonist that I can use to turn on either of these inhibitory pathways? Can I simply use recombinant PD-L1 as a way to generate PD-1 signaling or do I need to co-culture my human primary T cells with some type of PD-L1 expressing cell line?
Thank you in advance!