Does anyone use the reading of 495nm to assess cellular viability in Huh7.5 cells (MTT method)?

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To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

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Nikola M Stojanovic

We have used 540nm for MTT reading and I think it doesn't meter which type of cells are you using.

Sweta Kumari

I also used 540 nm for MTT assay and this assay is not cell line specific.

Johan Van Meerloo

540 or 560 nM is the most efficient,

i always substract a 720nM readout as a background control.

Irene Heredero Bermejo

We work with HeLa cells and read absorbance at 570 nm for MTT. It is not cell line specific as they have said.

Its the wavelength where the colour is giving the best od values.