What other reagent could be used as a positive control of the Griess method?

I use LPS at 5ug/mL as a positive control in the Griess method, but nitrite production is very low.

06 July 2019 4,009 4 View

I can use fluconazole for eliminating fungi in cultured cells?

09 October 2016 5,783 1 View

What mathematical formula is used to determine FFU/mL?

08 September 2015 3,074 1 View

Why do my VERO cells die after a while?

VERO cells die after a while. There isn't evidence of contamination (bacteria and fungi). In addition, other cell lines aren't killed and with the same culture medium VERO. Which can be?

02 March 2015 8,189 7 View

The SGR amplification curve in my qPCR does not appear. However, when I put the SGR qPCR product in agarose gel the amplicon appears. What happened?

SGR is a sample and PJFH1 is endogenous control. *The mixture was the same for both samples, changing only the primers.

02 March 2015 4,370 10 View

Has anyone of you managed to express plaque forming units (PFU) to dengue virus in Vero E6 or LLCMK2 cells?

I can not express plaque forming units (PFU) to dengue virus.

08 September 2014 9,434 4 View

Does anyone know the base sequences of human endogenous primers for real time PCR? Such as BAC1, HPRT and GAPDH.

Companies sell with probe, but I don't need the probe. How to get the base sequences of the endogenous primer?

01 February 2014 5,667 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View