Has anyone of you managed to express plaque forming units (PFU) to dengue virus in Vero E6 or LLCMK2 cells?

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André Luis da Costa-da-Silva

Hi Edgar,

We are producing Dengue plaque assay using LLCMK2 and focus assay using C6/36 cells. I did not understand why you can not express pfu.... Plaques are not forming?

Andre

Edigar Henrique Vaz Dias

Yes. Plaques are not forming. In c6/36 cells occurs the formation of generalized cytopathic effect.

Some Dengue genotypes do not form plaques. In this case, you should try other titration methods... Do you think this is the case? Or you already saw plaques with this strain... ?

I will try titration in VERO cells.