What other reagent could be used as a positive control of the Griess method?

I use LPS at 5ug/mL as a positive control in the Griess method, but nitrite production is very low.

06 July 2019 4,009 4 View

What mathematical formula is used to determine FFU/mL?

08 September 2015 3,074 1 View

Why do my VERO cells die after a while?

VERO cells die after a while. There isn't evidence of contamination (bacteria and fungi). In addition, other cell lines aren't killed and with the same culture medium VERO. Which can be?

02 March 2015 8,189 7 View

The SGR amplification curve in my qPCR does not appear. However, when I put the SGR qPCR product in agarose gel the amplicon appears. What happened?

SGR is a sample and PJFH1 is endogenous control. *The mixture was the same for both samples, changing only the primers.

02 March 2015 4,370 10 View

Has anyone of you managed to express plaque forming units (PFU) to dengue virus in Vero E6 or LLCMK2 cells?

I can not express plaque forming units (PFU) to dengue virus.

08 September 2014 9,434 4 View

Does anyone use the reading of 495nm to assess cellular viability in Huh7.5 cells (MTT method)?

MTT method

05 June 2014 9,844 5 View

Does anyone know the base sequences of human endogenous primers for real time PCR? Such as BAC1, HPRT and GAPDH.

Companies sell with probe, but I don't need the probe. How to get the base sequences of the endogenous primer?

01 February 2014 5,667 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

No title

28 February 2021 9,936 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View