Nikola M Stojanovic

11 Questions 163 Answers 0 Followers

Questions related from Nikola M Stojanovic

Can you suggest me fluorimetric method for saliva cortisol determination?

I have trouble finding adequate method for fluorimetirc determination of saliva cortisol. I don't want to use ELISA nor RIA. Can you help me? Also, do I have to extract cortisol from saliva? How...

06 June 2016 8,886 3 View

Can you suggest me the protocol for XTT assay?

I have XTT powered substance (not kit!) so I have problem finding a proper protocol for applying this in macrophage/lymphocyte cell cultures. I have looked at many papers and kit protocols where...

01 January 2015 4,919 5 View

Can anyone help me with cell isolation and culturing?

I have some experience in cell isolation and culturing them. However those are mostly short assays (1-2h) and no real stuff. I want to know what culture medium is best for macrophages,...

09 September 2014 6,534 20 View

Acetic acid induced writhings in mice?

I asked similar question some time ago and i want to try to get the help once more! During experiments with abdominal writhing induced with acetic acid very different results are obtained! In the...

08 August 2014 3,885 5 View

Behavior asymmetry coefficient?

These days I read in some article about behavior asymmetry coefficient that is calculated from parameters of open field test. However I didn't downloaded that paper. Now I can't find anything...

08 August 2014 2,791 10 View

Does anyone have mouse brain atlas by Paxinos and Franklin (2001)?

Paxinos G, Franklin KBJ. 2001. The mouse brain in stereotaxic coordinates. San Diego, CA: Academic.

07 July 2014 8,709 55 View

Has anyone had troubles with HID/AB staining?

After HID exposure, AB is binding or is bound much less than expected. The tissue in question is rat stomach.

05 May 2014 7,334 2 View

Can someone suggest method for determination of oxidised glutathione ?

But if it is possible spectrophotometric (not fluorescence)

05 May 2014 7,472 2 View

How to prepare a solution of silymarin suitable for i.p. application to rats?

What solvent, solution? What dose?

03 March 2014 2,522 4 View

Picrotoxin convulsions?

Could you suggest doses for induction of convulsions with picrotoxin?

12 December 2013 6,390 4 View

Did anyone have any troubles while performing acetic acid induced writhing test in mice?

Very different number of writhings among animals in group

12 December 2013 9,123 2 View