Does anyone use the reading of 495nm to assess cellular viability in Huh7.5 cells (MTT method)?

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Medium replacement interfering with cell culture blot results?

We utilize an immortal endothelial cell line and various agonists to observe their effects on Src phosphorylation. However, our protocols seem to induce high basal levels of Src phosphorylation....

17 June 2024 1,649 0 View

I have a simple question: can i use "displacement" instead of "shift" when i refer to XRD peaks?

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Z-axis alignment in a protein for z-axis constraints in Amber software?

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Analytical frequency calculation error in ORCA 5.0.3, does anyone know how to change the calculation algorithm?

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27 March 2024 8,609 2 View

Which factors, practices, and tools impact trust in online business negotiations?

We addressed such a question at Exploring The Missing Link Between Trust in Business Negotia...

14 March 2024 2,875 1 View

LiF impurity on XRD data after HCl/LiF etching of Ti3C2 MXene ?

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Can a medium containing 0.5% of FBS induce instant and drastic morphological changes in culture cells?

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10 January 2024 8,232 5 View

Should I include non significant correlation coefficient while calculating summary effect size in random Meta analysis?

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How do I search a name in Zoological Record today?

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How to exfoliate graphene in large areas (sides above 50 to 100 μm)?

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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Could it be a cell culture contamination?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why activated CAR-Jurkat cell could not kill targets?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

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Good Day, can I ask for your help on the questionnaire that I want to adopt?

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03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

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Nikola M Stojanovic

We have used 540nm for MTT reading and I think it doesn't meter which type of cells are you using.

Sweta Kumari

I also used 540 nm for MTT assay and this assay is not cell line specific.

Johan Van Meerloo

540 or 560 nM is the most efficient,

i always substract a 720nM readout as a background control.

Irene Heredero Bermejo

We work with HeLa cells and read absorbance at 570 nm for MTT. It is not cell line specific as they have said.