10 Questions 7 Answers 0 Followers
Questions related from Edigar Henrique Vaz Dias
I need to do a scanning electron microscopy but I don't have the osmium oxide, can some other reagent replace it?
01 November 2017 3,018 4 View
The trypsin can change the VERO receptors used by dengue virus infection.
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I need inactivate dengue virus to mouse inoculation. The heat-inactivation is efficient?
04 August 2016 3,882 8 View
I need isolate the CDV in cell culture.
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I need a viral lysate. There are some substance to it? Something that disrupts the lipid envelope.
21 July 2016 4,847 1 View
I need remove cell debris, however i don't know if can filter the dengue virus in 0.22 um filters.
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Aedes albopictus cell culture (strain C6/36) die when incubated with dengue virus. Already the mosquito does not die when infected with dengue. Why?
18 February 2016 9,899 3 View
What's the concentration of gentamicin that can be used on mammalian cells culture? This antibiotic isn't toxic to culture?
31 March 2015 3,156 4 View
I need to store the RSV for a long period. What is the better reagent to do this (glycerol or sucrose)? What is the concentration?
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MTT method
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