Nikola M Stojanovic

8 Questions 122 Answers 0 Followers

Questions related from Nikola M Stojanovic

How can I make LPS stock solution?

I have LPS (powder) at -20,but I'm planing to dissolve it and use it to induce cell activation (macrophage/fibroblast). How should I divide the stock solution? Can I keep those solutions at -20...

04 January 2016 5,607 19 View

How big are splenocytes?

I'm doing morphometric analysis of splenocytes and from the data that I have processed up to now I see quite strange numbers for Feret diameter and I wanna know how big are splenocytes? Some...

27 April 2015 1,543 9 View

Can you suggest me the protocol for XTT assay?

I have XTT powered substance (not kit!) so I have problem finding a proper protocol for applying this in macrophage/lymphocyte cell cultures. I have looked at many papers and kit protocols where...

11 January 2015 7,688 4 View

Can anyone help me with cell isolation and culturing?

I have some experience in cell isolation and culturing them. However those are mostly short assays (1-2h) and no real stuff. I want to know what culture medium is best for macrophages,...

21 September 2014 9,788 19 View

Behavior asymmetry coefficient?

These days I read in some article about behavior asymmetry coefficient that is calculated from parameters of open field test. However I didn't downloaded that paper. Now I can't find anything...

05 August 2014 7,961 9 View

Can someone suggest method for determination of oxidised glutathione ?

But if it is possible spectrophotometric (not fluorescence)

04 May 2014 6,127 2 View

Did anyone have any troubles while performing acetic acid induced writhing test in mice?

Very different number of writhings among animals in group

10 December 2013 1,624 2 View

Mouse model of EEG

Anyone interested in collaborating on evaluating the impact of various potential drugs on mouse brain electrical activity?

01 January 1970 4,392 0 View