10 Questions 152 Answers 0 Followers
Questions related from Nikola M Stojanovic
I have LPS (powder) at -20,but I'm planing to dissolve it and use it to induce cell activation (macrophage/fibroblast). How should I divide the stock solution? Can I keep those solutions at -20...
04 January 2016 5,707 19 View
I'm doing morphometric analysis of splenocytes and from the data that I have processed up to now I see quite strange numbers for Feret diameter and I wanna know how big are splenocytes? Some...
27 April 2015 1,647 9 View
I have XTT powered substance (not kit!) so I have problem finding a proper protocol for applying this in macrophage/lymphocyte cell cultures. I have looked at many papers and kit protocols where...
11 January 2015 7,791 4 View
Why is fetal calf serum necessary for this assay? When performed from bone marrow why do the cells need to be re-suspended in FCS? The methanol fixation is done after smears are made...so I don't...
18 October 2014 609 9 View
I have some experience in cell isolation and culturing them. However those are mostly short assays (1-2h) and no real stuff. I want to know what culture medium is best for macrophages,...
21 September 2014 9,887 19 View
These days I read in some article about behavior asymmetry coefficient that is calculated from parameters of open field test. However I didn't downloaded that paper. Now I can't find anything...
05 August 2014 8,061 9 View
I need to evaluate MIC activity of some plant extracts on C. difficile. Can anyone suggest a medium for C. difficile? Can something other than brain heart infusion be used? How long should...
10 July 2014 3,671 2 View
But if it is possible spectrophotometric (not fluorescence)
04 May 2014 6,209 2 View
Very different number of writhings among animals in group
10 December 2013 1,766 2 View
Anyone interested in collaborating on evaluating the impact of various potential drugs on mouse brain electrical activity?
01 January 1970 4,474 0 View
