Can "dirty" activated carbon (GAC) add contamination to water as it is being treated?

We have a system to treat water from an old industrial basin before discharging to the river. The system initially consisted of (in this order): a 3-stage bag filter, 3 granular activated carbon...

22 July 2019 9,298 3 View

Is it possible to mark shared first authors of a published article?

Is it possible to mark shared first authors or joint senior authors of published articles on your research profile?

04 April 2019 8,967 6 View

MYCOLOGISTS/BIOLOGISTS NEEDED: To help identify the fungal species pictured in the 5 following photographs?

My family and I would like to welcome any and all responses, advice, and any additional information that you would like to share. We would also like to thank you from the bottom of our hearts (in...

19 March 2019 2,640 5 View

How do I correct RNA pooling in wells during RNA Gel Electrophoresis?

I am running an agarose gel to check for RNA degradation, and I'm having trouble getting my sample to migrate out of the well. I've attached a copy of the picture showing the sample in the well....

19 February 2019 4,876 4 View

Method to determine low cellulose concentrations (0.5-1.5%) in feed?

I'm looking for a method that is sensitive enough to measure dietary cellulose content of rat feed at 0.5% cellulose, and up to 1.5%. We have tried the ADF method and AOAC 991.43, the ADF was...

21 May 2018 2,237 1 View

Malaysian version of the Rosenberg Self-Esteem Scale?

Dear Colleagues, I am looking for the Malay version of the RSES, preferably the one Prof. Viren Swami (2011) studied in his chapter: Further Examination of the Psychometric Properties of a Malay...

06 November 2017 6,494 2 View

I am looking for a method to stain spleen sections to identify germinal centers. Which stains and/or antibodies would you recommend?

26 September 2016 3,022 3 View

I have problems culturing rabbit hybridomas. Do they usually present slow growth?

Does anyone have specific techniques or media to help the cell line increase its growth?

31 August 2016 8,770 1 View

Does anyone know where I can get MDA-MB-231 LUC cells that are able to develop tumors in the subcutaneously in the flank of nude mice?

09 June 2016 9,437 2 View

Does anyone have information about the PCL-5 and the Centrality of Event Scale in Arabic?

15 October 2015 5,571 18 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View