Does anyone know of a kit or a protocol which allows me to test for bilirubin in cell lysates?

More Kimberly Jasmer's questions See All

Can I use candle-jar technique to lactic acid bacteria?

I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria

15 July 2024 8,890 2 View

Replacement for acetone in dehydratization of cryo sections?

We are currently trying to replace acetate when dehyrdating ("drying") cryo sections on glass slides with a less harmful alternative. For many chemical purposes ethyle lactate is recommended - has...

10 June 2024 5,213 3 View

Can frogs transmit blood parasites to humans?

Can frogs transmit blood parasites to humans? If yes, how? And if not, why?

03 April 2024 6,284 0 View

Is it possible to detect murine COX-2 protein at 50kDa via western blot?

Hi everyone, I've ran a western on mouse ileal lysates and probed for COX-2. 50ug was loaded per sample and the dilution factor was 1:500 for the primary (monoclonal mouse ab) and 1:10000 for the...

13 November 2023 6,849 0 View

Why would immortalized endometriotic epithelial cells peel off of wells?

As for my 12Z cell line, the cells grow very well and are tenacious to the wall of the plastic culture flasks. However, they do not adhere to the wells of plates properly after seeding the cells...

01 March 2023 2,423 0 View

How do you enhance the cell proliferation of immortalized endometriotic stromal cells (22b)?

For the immortalized endometriotic stromal cells (22b), we have maintained them on an array of media types, such as Dulbecco's Modified Eagle Medium. Nutrient Mixture F-12 with 10% fetal calf...

01 March 2023 4,805 2 View

Trichrome Stain in Bone- what is red and blue?

Hi, I am doing a freelance project on bone defects treated with control and treated bone sponge- so I know little about the actual study and bone is not my forte in science. I'm told the...

10 February 2023 2,892 1 View

Fiber Photometry - how to keep caps from detaching?

I am recently new in trying my hand at fiber photometry, but recently implanted fiber optics as well as viruses to monitor both dopamine and serotonin neurotransmission. I successfully implanted...

11 December 2022 8,954 1 View

How to extract DNA from buccal swabs in preservative?

I have some buccal swab samples that are in a liquid preservative. Our group is trying to extract the DNA from these samples using the E.Z.N.A Tissue DNA kit and Forensic DNA kit. Both protocols...

26 January 2022 9,843 1 View

Why is my bis-tris gel not resolving well?

Hello all! I am struggling to understand why my bis-tris gel is not resolving well. There is not a uniform band forming in the lanes causing the gel to turn completely blue. I am using this...

20 December 2021 2,889 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View