Hi Jia, you can get cDNA of RNA by using RT primer and reverse transcriptase which has ability to replace U by T when it builds cDNA. Hope, that would help.

Thanks, but you talked about regular RT-PCR that does not amplify RNA signals at RT but at PCR. I wanted to also amplify RNA signals at RT stage before PCR stage.

You can extract RNA, then convert to cDNA and then amplified by PCR. You can then measure the levels of amplified cDNA by fluorescence in real time during the exponential phase of amplification. Best wishes