8 Questions 21 Answers 0 Followers
Questions related from Jia-Wang Wang
How many microRNAs can be detected in human plasma or serum by PCR and next generation sequencing?
06 June 2018 2,518 3 View
We have used Premix Ex Taq (Probe qPCR) Master Mix. Takara Bio Premix Ex Taq (Probe qPCR) for awhile, but recently found that it didn't give satisfactory results, so we switched to TaqMan™...
04 April 2018 6,351 2 View
Using the same probe and on the same PCR plate, both abnormal Taqman qPCR multicomponent plot normal Taqman qPCR multicomponent plot were observed. The only difference between the two is the...
02 February 2018 9,132 4 View
Does anyone know if one can linearly amplify an RNA to cDNA by a reverse transcription primer and Tth DNA polymerase, which has a potent transcriptase activity at the presence of Mn?
12 December 2017 5,925 3 View
I saw a website that the estimated titers of adenoviruses in the supernatant snd pellet collected from a 10 cm dish. The information is very useful, but I couldn't find this website again. Would...
04 April 2015 2,817 2 View
What is the maximum amount of DNA that can be inserted into the genome of mouse fertilized eggs using CRISPR/Cas for Homologous Recombination (HR)?
01 January 2015 5,796 9 View
We got almost 100% transfection efficiency by Lipofectamine 2000. Now it is low, probably only 30 to 50%. Just wondering if the cells have been contaminated with mycoplasma, affecting the...
08 August 2014 1,217 6 View
The two Marraffini Lab CRISPR Plasmids for bactera genome editing have a spacer cloning site, just wondering why you need two spacers. Do you know if there is a plasmid that can express both Cas9...
08 August 2014 1,480 7 View
