Any advice on selecting human or animal studies to conduct a systematic review?

Human studies are more relevant to the project, however there are more mouse studies because it is mostly carried on neuron progenitor cells to identify the effect of a virus in the development of...

11 January 2021 5,628 3 View

Any papers discussing the effect of Zika virus on neuron development and its effects on neuronal functions?

I am looking into this research, any interesting papers relating to this would be amazing. Papers on pregnant women and infants would be great. Thank you :)

30 December 2020 1,962 3 View

I need updated CTE Sports related research?

I am looking for epidemiologyCTE for 2019 or 2020. also looking for treatment/management studies studies identifying tau pathology on postmortem studies. I have a few but struggling to find more...

28 October 2020 624 3 View

What PCR and real-time PCR machines perform best?

Hi everyone, I am equipping a new molecular biology/microbiology laboratory and I need to purchase a PCR and/or a real-time PCR machine. So I'm looking for suggestions. Is there a new model that...

11 October 2020 9,791 17 View

Why is anhydrous acetonitrile used to dissolve amidites in the synthesis of oligo nucleotide in solid phase?

Hi, anybody help me? Why is anhydrous acetonitrile used to dissolve amidites in the synthesis of oligonucleotide in solid phase? What's the consequences?

28 July 2020 7,993 3 View

What the best way to improve Illumina genomic assembly with nanopore metagenomic data?

I have the Illumina reads and assembly of my bacteria from pure culture DNA sequencing and the nanopore sequencing data from the metagenome (DNA of co-culture). Does anyone know the best way to...

14 July 2020 6,516 3 View

Which phenomenological mathematical models describing the dynamic behaviour of microorganisms in the same ecosystem seem to be the most widely used ?

Hi, As part of a year-round trinomial project, I am looking for information about phenomenological mathematical models describing the dynamic behaviour of several microorganisms in the same...

01 April 2020 9,365 2 View

How do I describe a SDS PAGE when i am specifically looking for p53 protein?

Do I look at the most prominent bands on the figure? or do I look at bands at level 53kDa, even though they are not the most prominent in the image? Would i also mention that multiple bands...

23 March 2020 2,445 3 View

Flow cytometry: How to apply compensation to environmental samples when using an e.coli antibody?

We are using flow cytometry to run e.coli antibodies in environmental water samples. What is the best way to apply compensation? Thanks!

10 March 2020 8,379 4 View

Model protein that I can use as replacement for antibodies ?

Buying antibodies for lab tests is expensive, therefore I would like to know which protein model I could use instead. Thank you in advance.

23 January 2020 4,077 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

QTL, recombination frection?

22 February 2021 2,588 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

16 February 2021 9,050 5 View

Is Genetic Algorithm usage for Motor Conception FEA optimization a good choice?

Hi RG community, Like the title shows ,i was wondering if choosing the GA for this kind of Optimizaiton is a good choice? For example we have a population of 100 (for 7-8 Optimization Variables),...

14 February 2021 1,160 7 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

What can explain a low expected heterozygosity rate for T. monococcum wild samples (0.09) ?

I conducted an SDS PAGE electrophoresis on wheat wild varieties to separate their glutenins and measure the genetic diversity of my sample. I only ran one electrophoresis. Can a genetic drift...

14 February 2021 3,971 1 View