Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Can I perform Zeta-potential analysis with for nanoparticles dispersed in propylene glycol using disposable capillary cell (DST1070) ?

I am conducting a zeta-potential experiment but it shows I have a poor quality result and my sample conductivity is low. My sample is copper nanoparticles dispersed in propylene glycol.

05 December 2020 3,966 9 View

No title

02 July 2020 1,966 6 View

Please help; How can I address ontological, epistemological, axiological issues in my research?

I am studying an application of Competence Based Training by teachers. I want to know how they apply it in classrooms, if they have any Pedagogy Content Knowledge (PCK) deficiency in using such a...

05 May 2020 1,039 15 View

Why is my antenna showing an impedance of 106.6 Ohms on the smith chart instead of 50 Ohms?

Hello everyone, I'm having difficulty with the line input impedance of a patch antenna signal. It's showing s1,1 impedance of 117 ohms on the smith chart but the correct impedance should be 50...

16 March 2020 6,552 3 View

Is there a statistical distribution to model the sum of independent squared Gaussian random variables with different means and variances?

The sum of squared zero-mean Gaussian random variables with different variances can be modeled by a generalized Chi-square distribution. However, I am looking for the PDF of the sum of squared...

06 January 2020 2,001 6 View

What mathematical function or formulae can enable me superimpose multiple curves (up to 25 ) on one another?

I wish to shift multiple lines or curves (up to 25 lines/curves) so that they are superimposed on one another. This is to enable me see clearly the points or regions where any one of the curves...

25 November 2019 6,530 4 View

Binary online matrix calculator?

Please could someone assist with sites of an online matrix binary calculator

19 November 2019 5,522 3 View

How to model a hyperelastic plate in ansy apdl?

I am modelling an elastomeric bearing in ansys apdl but the elastomer does not bulge under compressive load as it should. it "splashes out" under any significant loading. is it the loading problem...

26 June 2019 2,003 3 View

What is a good measure of surface roughness (i.e. topographic complexity) that can be derived from a DEM?

I want to generate an environmental layer depicting topographic roughness, or complexity, scores using a DEM in ArcMap. I know there are lots of available methods (i.e....

09 April 2019 6,563 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View