I loaded the DNA with its respective buffer load in one lane. Then, in the other lane I loaded the molecular weight marker and ran the electrophoresis. When I do the disclosure in the transilluminator I cannot observe the bands in any lane.
Sofiane Benyamina
Hello,
Are you sure that you added EThidium Bromide (product to be added with adequate protection, it is dangerous and causes damage to DNA) to be able to visualize?
If so, perhaps you left the migration on electrophoresis gel for a very long time which caused you to lose your DNA samples which came out of the agraose gel.
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Nelufar Yasmen
Make sure your cDNA synthesis process is correct. Also the loading dye.
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