Does anyone have simplified methods for plant protein content determination using Bradford assay?

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How to solve this error while I simulate gidl using tcad ?

There is an error message "interface silicon/oxide not found in grid xxx.tdr file"

26 June 2024 5,551 0 View

How to eliminate imaginary phonon frequency in phonon dispersion of Coved Graphene Nanoribbons?

Dear all, I want to calculate the phonon dispersion of 4CNR-1-0 (structure is in Fig. 2 (b) in this article Designing coved graphene nanoribbons with charge carrier mob... ) and then calculate...

17 June 2024 6,911 2 View

Synthesis poly(N-isopropylacrylamide) through ATRP by using CUBr/PMDETA?

Monomer: NIPAM; Initiator: BiBB; Catalyst: CuBr; Ligand: PMDETA I added CuBr and the mixture was blue, after that, I purged with nitrogen for 30 minutes and the mixture turned green. The mixture...

27 May 2024 9,144 1 View

I cultured microorganisms obtained from the soil on a medium. Can you identify what kind of microorganisms these are?

I cultured microorganisms obtained from the soil on a medium. Can you identify what kind of microorganisms these are? Is it possible that this microorganism is Stenotrophomonas panacihumi?

22 May 2024 875 4 View

How to determine decision limit (CCα) according to CIR 2021/808?

CIR 2021/808 written as "the CCα shall be calculated as follows: Method 1: by the calibration curve procedure according to ISO 11843-1:1997 ( 14) (here referred to as critical value of the net...

13 May 2024 9,481 0 View

Time to vacuum the chamber of the E-beam evaporation system?

We bought the E-beam evaporation system without a loading chamber. until now, we have used this system about 20~30 times for the deposition of Al2O3, Ti, Au, and Al having thicknesses from 50~100...

23 April 2024 5,156 4 View

Testing methods to determine a non-gene-silencing enzyme inhibitor?

I've recently been trying to investigate the inhibition of H2S-synthesising enzymes: CBS, CSE and 3-MST, via PCR. We've ordered EGCG, PAG and i-3MT-3 to inhibit each enzyme respectively, and have...

16 March 2024 392 1 View

What are the statistical approach for servant leadership in F&B?

To have multiple case study exploration among Malaysia restaurants that study the impact of servant leadership, what are the best statistical approaches for the case studies?

01 March 2024 2,737 1 View

How to reduce the collapse of microfluidic chamber?

During the fabrication of the microfluidic chamber (check its structure in the file, a 5x2.4mm microfluidic chamber with height of 20 μm), the ceilings of the chamber often collapse when bonding...

27 February 2024 6,330 3 View

Why it is necessary to dilute the concentration of surfactant during the process of crosslinking of droplet microfluidic produced calcium alginate?

I see in some references like doi: 10.1021/acs.analchem. 1c02335 and /10.1016/j.colsurfb.2022.113114, mention that it is necessary to dilute the concentration of surfactant during the process of...

27 February 2024 9,571 2 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View