Eyu Chan Hong

8 Questions 15 Answers 0 Followers

Questions related from Eyu Chan Hong

Does anyone have simplified methods for plant protein content determination using Bradford assay?

I am doing plant tissue culture, and the papers I found most are animal protein based... I am not sure whether it is applicable to plant as well... Does anyone has simplified method for protein...

21 February 2019 5,780 3 View

Does anyone know how to tissue culture moss without contamination?

I had tried surface sterilize the moss but it came out with fungal contamination...high concentration of surfactant turned out yellowed moss and dead...does anyone tried before?

17 February 2019 1,628 1 View

How to prepare 75 uM nitro blue tetrazolium HCl prior to superoxide dismutase (SOD) assay?

Does anyone know how to prepare nitro blue tetrazolium solution at 75 uM? Some websites suggested 70 % dimethlformamide (DMF) solution or ethnaol... I wonder the solvent can be used or not?...

24 January 2019 5,531 7 View

I recently came across testing on antioxidant activities of plants treated with fluorescent and LED. What can I conclude from the experiment?

LED treated plants gave lower concentrations on APX, SOD and CAT antioxidant activities than fluorescent light...what does it tell from the concentration of the enzymes? which is better?

06 June 2018 4,532 6 View

Safranin-Fastgreen staining often troublesome due to the fact that fastgreen will counterstain safranin. Any alternatives?

The thickness and timing in staining is very crucial in histology. I use 6 micrometer thick sample and sometimes fast green just need a dip about 3 seconds before it actually over stain the whole...

05 June 2018 4,240 3 View

How to carry out experiment with replicate at least 30 with two variables (Hormone for example) at different concentrations?

One-way ANOVA requires n=30. However, of two hormones (BAP and 2-iP) tested at different concentrations (0, 1, 2, 3, and 4 mg/L), in order to achieve 30 samples or replicates, we can do many ways...

09 March 2018 9,213 3 View

Response Surface Methodology (RSM) is usually used by researcher to find out the optimised combination of PGR used in Plant Tissue Culture.

Does RSM can be done using SPSS ver 22.0? Does anyone has simplest procedure to analyse data using RSM?? What are the other analyses that can be used to obtain the optimised data instead of...

14 October 2016 860 3 View

When we were going to prepare stock solution for PGR (filter sterilized) such as TDZ and Zeatin, do we need to measure the pH of the stock?

Because if we don't measure the pH of the stock solution, means the stock solution that will be added into autoclaved media will change the pH of media. If we did measured the pH of stock, how...

07 October 2016 207 5 View