I am going to start MIN6 culture. I have never work cells other than iPS and hepatocytes. Could you please share your know-how and tricks to handle the type of cell (passaging time, optimal condition, etc.)
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
In dentistry, we sometimes need ‘binocular diplopia’ when dealing with complex dental problems, which means using different angles and tools to ensure the treatment is effective. In your opinion,...
09 July 2024 3,898 0 View
Lorsque nous nous posons cette question , nous nous basons sur un fait et constat très simple : Dans les manuels d’apprentissage du chinois, qui tiennent lieu de programme pour l’instant dans les...
08 July 2024 7,650 3 View
Hi everyone! I am using a Q5 high fidelity DNA polymerase to amplify a 6.7 kb plasmid. The PCR with Q5 went well with longer primers (~55 bases) at 70-72 degree C. However, I keep failing in the...
17 June 2024 1,734 4 View
The question is based on the article “Tenets of Specimen Management in Diagnostic Microbiology” by Rajeshwar and Pathak. It explores how advances in technology, specifically barcoding systems, can...
10 April 2024 9,863 1 View
Hi! I got divorced so I changed back to my maiden name. How to add to my profile? It’s now Grace van Leeuwen/ Grace Caroline van Leeuwen
26 February 2024 3,482 1 View
I am in the process of learning how to culture MSCs that I have flushed from the bone marrow of mice femurs. So far, the process runs smoothly until I attempt to trypsinize the cells, I attempt to...
21 February 2024 9,211 0 View
the concept is derived from Sundareshan & Khardori (2019) regarding the usage of antibiogram as Public Health Tools to Slow Down Antibiotic Resistance. it is indicated in the article that...
06 February 2024 2,293 1 View
Hi, I performed a competition assay of serially diluted concentrations of the unlabeled peptide by adding a constant concentration of the FITC-labeled peptide. I have observed higher fluorescence...
06 January 2024 6,949 6 View
. i need articles that can help me find this information
08 December 2023 5,734 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
I am trying to analyse data from a survey examining what variables affect teachers perceived barriers to incorporating technology into their classroom. I have 5 predictor variables however my DV...
06 August 2024 1,752 3 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View